Department of Biology and Environmental Science, Allama Iqbal Open University, Islamabad, Pakistan; Department of Plant Sciences, Quaid-i-Azam University, Islamabad, Pakistan.
Department of Biology and Environmental Science, Allama Iqbal Open University, Islamabad, Pakistan.
Genomics. 2020 Nov;112(6):4760-4768. doi: 10.1016/j.ygeno.2020.07.031. Epub 2020 Jul 24.
The plant microbiome influence plant health, yield and vigor and has attained a considerable attention in the present era. In the current study, native bacterial community composition and diversity colonizing Triticum aestivum L. rhizosphere at two distant geographical locations including Mirpur Azad Kashmir and Islamabad was elucidated. Based on IonS5™XL platform sequencing of respective samples targeting 16S rRNA gene that harbor V3-V4 conserved region revealed 1364 and 1254 microbial operational taxonomic units (OTUs) at ≥97% similarity and were classified into 23, 20 phyla; 70, 65 classes; 101, 87 orders; 189,180 families; 275, 271 genera and 94, 95 species. Respective predominant phyla accounting for 97.90% and 98.60% of bacterial community were Proteobacteria, Actinobacteria, Acidobacteria, Bacteroidetes, Firmicutes, Chloroflexi and Gemmatimonadetes. Diversity indices revealed variations in relative abundance of bacterial taxa owing to distant geographical locations however predominant bacterial taxa at both locations were similar. These findings paved a way to dissect consequence of associated microbiota on future wheat production system.
植物微生物组影响植物的健康、产量和活力，在当今时代受到了相当的关注。本研究阐明了在两个不同地理位置（包括米尔布尔阿扎德克什米尔和伊斯兰堡）的小麦根际定殖的本地细菌群落组成和多样性。基于针对 16S rRNA 基因的 IonS5XL 平台测序，分别对各自的样本进行了测序，该基因包含 V3-V4 保守区，揭示了在≥97%相似性水平上的 1364 和 1254 个微生物操作分类单元（OTUs），并分为 23、20 门；70、65 纲；101、87 目；189、180 科；275、271 属和 94、95 种。分别占细菌群落 97.90%和 98.60%的主要菌门为变形菌门、放线菌门、酸杆菌门、拟杆菌门、厚壁菌门、绿弯菌门和芽单胞菌门。多样性指数表明，由于地理位置的不同，细菌类群的相对丰度存在差异，但两个地点的主要细菌类群相似。这些发现为剖析相关微生物群对未来小麦生产系统的影响铺平了道路。
