UMR Transfrontalière BioEcoAgro N° 1158, Univ. Lille, INRAE, Univ. Liège, UPJV, YNCREA, Univ. Artois, Univ. Littoral Côte d'Opale, ICV - Institut Charles Viollette, F-59000, Lille, France.
Institute of Nutrition and Functional Foods (INAF), Dairy Research Center (STELA), Department of Food Sciences, Pavillon Paul-Comtois, 2425, rue de l'Agriculture, Université Laval, Québec, G1V 0A6, Canada.
Probiotics Antimicrob Proteins. 2021 Feb;13(1):208-217. doi: 10.1007/s12602-020-09687-y.
Bacteriocin-producing Escherichia coli ICVB442, E. coli ICVB443, Enterococcus faecalis ICVB497, E. faecalis ICVB501, and Pediococcus pentosaceus ICVB491 strains were examined for their pathogenic risks and compatibility and hence suitability as consortium probiotic bacteria. Except for E. coli ICVB442, all were inclined to form biofilm. All were gelatinase-negative, sensitive to most of the antibiotics tested and not cytotoxic to porcine intestinal epithelial cells (IPEC-1) when tested at a multiplicity of infection (MOI) of 1. P. pentosaceus ICVB491 stood apart by inhibiting the other four strains. Both E. coli strains and E. faecalis ICVB497 strain were β-hemolytic. Survival in the TIM-1 dynamic model of the human digestive system was 139% for the tested E. coli ICVB443 strain, 46% for P. pentosaceus ICVB491, and 32% for the preferred E. faecalis ICVB501 strain. These three potential probiotics, which are bacteriocin-producing strains, will be considered for simultaneous use as consortium with synergistic interactions in vivo on animal model.
产细菌素的大肠杆菌 ICVB442、大肠杆菌 ICVB443、粪肠球菌 ICVB497、屎肠球菌 ICVB501 和戊糖片球菌 ICVB491 菌株被检测其致病风险和相容性，从而确定它们作为共生益生菌的适用性。除了大肠杆菌 ICVB442，所有菌株都倾向于形成生物膜。所有菌株均为明胶酶阴性，对大多数测试的抗生素敏感，当感染复数 (MOI) 为 1 时，对猪肠上皮细胞 (IPEC-1) 无细胞毒性。戊糖片球菌 ICVB491 株与众不同之处在于抑制其他四种菌株。两种大肠杆菌菌株和粪肠球菌 ICVB497 菌株均为β-溶血。在人体消化系统的 TIM-1 动态模型中，测试的大肠杆菌 ICVB443 菌株的存活率为 139%，戊糖片球菌 ICVB491 为 46%，首选的屎肠球菌 ICVB501 为 32%。这三种具有协同作用的潜在益生菌（产细菌素菌株）将被考虑同时用于动物模型体内的协同作用。
