Breast Cancer Center, the Third Affiliated Hospital of Sun Yat-sen University, Guangzhou, 510000, P.R. China.
Department of Pathology, the Third Affiliated Hospital of Sun Yat-sen University, Guangzhou510000, P. R. China.
Int J Med Sci. 2020 Jul 6;17(12):1763-1772. doi: 10.7150/ijms.48162. eCollection 2020.
Gastric cancer (GC) is a common malignancy throughout the world. Biomarkers for prognosis and risk evaluation of GC are rapidly discovered. We investigated the prognostic role of FLAD1, an important protein-coding gene that affects cell cycle and survival. The expression of FLAD1 at mRNA levels in GC tumor tissues and normal tissues was mined and analyzed in Oncomine database and verified in 10 pairs of GS tissues and their adjacent normal tissues in our center by RT qPCR. The FLAD1 protein expression were detected in 106 paraffin-embedded GC tissues by immunohistochemistry (IHC). Statistical analyses were applied to evaluate the clinical significance of FLAD1. The prognostic value of FLAD1 mRNA expression was also analyzed using the Kaplan-Meier plotter (www.kmplot.com). Statistics obtained from online database suggested FLAD1 mRNA was overexpressed in GC tissues. The results were further validated in 10 pairs of GS tissues and adjacent normal tissues in our center (p=0.021). IHC and survival analysis of GC samples from 106 patients showed FLAD1 was overexpressed in 63/106 (59.4%) patients and was associated to higher TNM stage (p=0.026). Multivariate analysis revealed FLAD1 was an independent prognostic factor for GC (p < 0.001). Furthermore, FLAD1 mRNA was associated to unfavorable overall survival (OS), first progression (FP), and post-progression survival (PPS) of GC (p<0.001). FLAD1 in GC is overexpressed at both mRNA and protein level and could be a potential biomarker for GC prognosis.
胃癌（GC）是一种常见的恶性肿瘤。目前正在快速发现用于 GC 预后和风险评估的生物标志物。我们研究了 FLAD1 的预后作用，FLAD1 是一种影响细胞周期和存活的重要蛋白质编码基因。在 Oncomine 数据库中挖掘并分析了 GC 肿瘤组织和正常组织中 FLAD1 的 mRNA 水平表达，并通过 RT-qPCR 在我们中心的 10 对 GS 组织及其相邻正常组织中进行了验证。通过免疫组织化学（IHC）检测了 106 例石蜡包埋的 GC 组织中 FLAD1 蛋白的表达。应用统计学分析评估了 FLAD1 的临床意义。还使用 Kaplan-Meier plotter（www.kmplot.com）分析了 FLAD1 mRNA 表达的预后价值。来自在线数据库的统计数据表明，FLAD1 mRNA 在 GC 组织中过表达。结果在我们中心的 10 对 GS 组织及其相邻正常组织中得到进一步验证（p=0.021）。对 106 例 GC 样本的 IHC 和生存分析表明，FLAD1 在 63/106（59.4%）例患者中过表达，并且与更高的 TNM 分期相关（p=0.026）。多变量分析显示，FLAD1 是 GC 的独立预后因素（p<0.001）。此外，FLAD1 mRNA 与 GC 的不良总生存（OS）、首次进展（FP）和进展后生存（PPS）相关（p<0.001）。GC 中 FLAD1 在 mRNA 和蛋白水平均过表达，可能是 GC 预后的潜在生物标志物。
