Yang Qin, Meng Ju, Zhang Wei, Liu Lu, He Laping, Deng Li, Zeng Xuefeng, Ye Chun
School of Liquor and Food Engineering, Guizhou University, Guiyang, China.
Guizhou Provincial Key Laboratory of Agricultural and Animal Products Storage and Processing, Guiyang, China.
Front Microbiol. 2020 Jul 2;11:1130. doi: 10.3389/fmicb.2020.01130. eCollection 2020.
The formation of biogenic amines (BAs) is an important potential risk in Suan yu. This study investigated the amine production abilities of 97 strains of enteric bacteria screened from Suan yu. The genotypic diversity of amino acid decarboxylase and the effect of pH were explored on 27 strains of high-yield BAs. Results showed that high levels of putrescine, histamine, and cadaverine were produced by the 97 strains. In addition, 27 strains carried , , , , and genes. Thirteen carried gene. 42C2 produced the highest putrescine content of 880 mg/L via the ornithine decarboxylase pathway. The highest histamine content was produced by 13C2 (1,869 mg/L). The highest cadaverine content was shown by 47C2 (1,821 mg/L). , , , , and play important roles in the cellular acid stress response. Acid stress caused the growth delay but can increase the contents of putrescine, histamine, and cadaverine. Decarboxylase was strain specific rather than species specific. This study provides a reference for the genotypic diversity of decarboxylase and effect of pH on the types and abilities of BAs produced by enteric bacteria in Suan yu.
生物胺（BAs）的形成是酸鱼中一个重要的潜在风险。本研究调查了从酸鱼中筛选出的97株肠道细菌的胺产生能力。对27株高产BAs的菌株，探讨了氨基酸脱羧酶的基因型多样性及pH的影响。结果表明，97株菌株产生了高水平的腐胺、组胺和尸胺。此外，27株菌株携带了、、、、和基因。13株携带基因。42C2通过鸟氨酸脱羧酶途径产生的腐胺含量最高，为880 mg/L。13C2产生的组胺含量最高（1869 mg/L）。47C2显示出最高的尸胺含量（1821 mg/L）。、、、、和在细胞酸应激反应中起重要作用。酸应激导致生长延迟，但可增加腐胺、组胺和尸胺的含量。脱羧酶具有菌株特异性而非物种特异性。本研究为酸鱼中肠道细菌产生BAs的脱羧酶基因型多样性及pH对其类型和能力的影响提供了参考。
