Sun Zhenxiao, Zhang Yi, Lin Xinping, Zhang Sufang, Chen Yingxi, Ji Chaofan
School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, China.
State Key Laboratory of Marine Food Processing and Safety Control, Dalian Polytechnic University, Dalian 116034, China.
Foods. 2023 Sep 29;12(19):3625. doi: 10.3390/foods12193625.
, a spoilage bacterium in fermented foods, produces harmful biogenic amines (BAs). Although is widely used to inhibit spoilage bacteria, the inhibition pattern and inhibition mechanism of by are not well studied. In this study, we analysed the effects of the addition of cell-free supernatant (CFS) on the growth and BA accumulation of and revealed the mechanisms of changes in different BAs by using RNA sequencing transcriptome analysis. The results showed that CFS could significantly inhibit BAs in a weak acid environment (pH 6), and the main changes were related to metabolism. Carbohydrate and energy metabolism were significantly down-regulated, indicating that CFS inhibited the growth activity and decreased the BA content of . In addition, the change in histamine content is also related to the metabolism of its precursor amino acids, the change in putrescine content may also be related to the decrease in precursor amino acid synthesis and amino acid transporter, and the decrease in cadaverine content may also be related to the decrease in the cadaverine transporter. The results of this study help to inhibit the accumulation of harmful metabolites in fermented foods.
一种发酵食品中的腐败细菌会产生有害的生物胺（BAs）。尽管[具体物质]被广泛用于抑制腐败细菌，但[具体物质]对[目标细菌]的抑制模式和抑制机制尚未得到充分研究。在本研究中，我们分析了添加[具体物质]无细胞上清液（CFS）对[目标细菌]生长和BA积累的影响，并通过RNA测序转录组分析揭示了不同BAs变化的机制。结果表明，[具体物质]CFS在弱酸环境（pH 6）中能显著抑制[目标细菌]的BAs，且主要变化与代谢有关。碳水化合物和能量代谢显著下调，表明[具体物质]CFS抑制了[目标细菌]的生长活性并降低了其BA含量。此外，组胺含量的变化也与其前体氨基酸的代谢有关，腐胺含量的变化可能也与前体氨基酸合成和氨基酸转运体的减少有关，尸胺含量的减少可能也与尸胺转运体的减少有关。本研究结果有助于抑制发酵食品中有害代谢物的积累。
