Institute of Zoology, Chinese Academy of Sciences, Beijing, China.
Genetic Research Center, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.
Biomed Res Int. 2020 Jul 2;2020:5217039. doi: 10.1155/2020/5217039. eCollection 2020.
Accumulating evidence so far has shown that EBV's miRNAs have been found to be involved in cancer progression. However, the comprehensive EBV miRNA expression profiles and their biological significance in EBV-associated diseases are not well documented. A comprehensive profiling of EBV-encoded miRNAs expressed in CAEBV, EBV-HLH, and nasopharyngeal carcinoma (NPC) patients was constructed, and the results showed that miR-BART19-3p was upregulated in all these diseases. Ectopic expression of miR-BART19-3p induced EBV-negative cell proliferation and suppressed cell apoptosis. Molecularly, adenomatous polyposis coli (APC) was identified to be a direct target of miR-BART19-3p, and APC mRNA expression was inversely correlated with miR-BART19-3p in CAEBV samples. Our results demonstrated that miR-BART19-3p contributes to the tumorigenesis of EBV-associated diseases and may be a potential therapeutic target.
迄今为止，越来越多的证据表明，EBV 的 miRNA 已被发现参与癌症的进展。然而，EBV 相关疾病中 EBV miRNA 的综合表达谱及其生物学意义尚未得到充分证实。本研究对 CAEBV、EBV-HLH 和鼻咽癌（NPC）患者中表达的 EBV 编码 miRNA 进行了全面分析，结果表明 miR-BART19-3p 在所有这些疾病中均上调。miR-BART19-3p 的异位表达诱导 EBV 阴性细胞增殖并抑制细胞凋亡。分子水平上，腺瘤性结肠息肉病基因（APC）被鉴定为 miR-BART19-3p 的直接靶标，CAEBV 样本中 APC mRNA 表达与 miR-BART19-3p 呈负相关。我们的研究结果表明，miR-BART19-3p 有助于 EBV 相关疾病的肿瘤发生，可能是一个潜在的治疗靶点。
