Galaz Jose, Romero Roberto, Xu Yi, Miller Derek, Levenson Dustyn, Para Robert, Varrey Aneesha, Hsu Richard, Tong Anna, Hassan Sonia S, Hsu Chaur-Dong, Gomez-Lopez Nardhy
Perinatology Research Branch, Division of Obstetrics and Maternal-Fetal Medicine, Division of Intramural Research, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, U.S. Department of Health and Human Services, Bethesda, MD, and Detroit, MI,USA.
Office of Women's Health, Integrative Biosciences Center, Wayne State University, Detroit, MI, USA; Department of Obstetrics and Gynecology, Wayne State University School of Medicine, Detroit, MI, USA; and Department of Physiology, Wayne State University School of Medicine, Detroit, MI, USA.
J Perinat Med. 2020 Sep 25;48(7):665-676. doi: 10.1515/jpm-2020-0037.
Objectives A sonographic short cervix is one of the strongest predictors of preterm delivery. However, the cellular immune composition of amniotic fluid in women with a short cervix has not yet been described. Herein, we determined cellular and soluble immune responses in amniotic fluid from pregnant women with a mid-trimester asymptomatic short cervix. Methods Amniotic fluid samples (n=77) were collected from asymptomatic women with a cervical length between 15 and 25 mm (n=36, short cervix) or ≤15 mm (n=41, severely short cervix) diagnosed by ultrasound. Flow cytometry and multiplex measurement of cytokines/chemokines were performed. Results (1) The cellular immune composition of amniotic fluid did not differ between women with a severely short cervix (≤15 mm) and those with a short cervix 15-25 mm; (2) amniotic fluid concentrations of multiple cytokines/chemokines were higher in women with a severely short cervix (≤15 mm) than in those with a short cervix 15-25 mm; (3) the cellular immune composition of amniotic fluid did not differ between women with a severely short cervix (≤15 mm) who ultimately underwent preterm delivery and those who delivered at term; and (4) amniotic fluid concentrations of IL-2, but not other immune mediators, were increased in women with a severely short cervix (≤15 mm) who ultimately delivered preterm compared to those who delivered at term. Conclusions Women with a severely short cervix (≤15 mm) have increased concentrations of pro-inflammatory mediators in the amniotic cavity; yet, these do not translate to changes in the cellular immune response.
超声检查发现的短宫颈是早产最强的预测指标之一。然而，短宫颈女性羊水中的细胞免疫组成尚未见报道。在此，我们确定了孕中期无症状短宫颈孕妇羊水中的细胞和可溶性免疫反应。方法：从超声诊断为宫颈长度在15至25毫米之间（n = 36，短宫颈）或≤15毫米（n = 41，严重短宫颈）的无症状女性中收集羊水样本（n = 77）。进行流式细胞术和细胞因子/趋化因子的多重检测。结果：（1）严重短宫颈（≤15毫米）的女性与短宫颈15 - 25毫米的女性羊水中的细胞免疫组成无差异；（2）严重短宫颈（≤15毫米）的女性羊水中多种细胞因子/趋化因子的浓度高于短宫颈15 - 25毫米的女性；（3）最终发生早产的严重短宫颈（≤15毫米）的女性与足月分娩的女性羊水中的细胞免疫组成无差异；（4）与足月分娩的女性相比，最终早产的严重短宫颈（≤15毫米）的女性羊水中IL - 2的浓度升高，但其他免疫介质未升高。结论：严重短宫颈（≤15毫米）的女性羊膜腔内促炎介质浓度升高；然而，这些并未转化为细胞免疫反应的变化。
