Department of Otorhinolaryngology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China.
Department of Infectious Diseases, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China.
PLoS One. 2020 Jul 27;15(7):e0236678. doi: 10.1371/journal.pone.0236678. eCollection 2020.
Head and neck squamous cell carcinoma (HNSCC), a tumor included oral cavity, lips, larynx, oropharynx, and the nasopharynx et al. The cell division cycle-associated (CDCA) protein family (CDCA1-8) critical for normal cell function and cancer cell proliferation. We explored the mutation signatures and expression levels of various CDCAs in detail in HNSCC. A comprehensive bioinformatics analysis pipeline based on copy number and gene expressions data from patients with HNSCC in order to given new insights into the possible functions and distinct prognostics that underlie CDCAs regulation. We compared the transcriptional expression of CDCAs in HNSCC and found significantly elevated mRNA expression of CDCA1-8 in HNSCC tissues across multiple datasets. We also found CDCA5/6/8 are over-expressed both transcriptionally and translationally in patients with HNSCC. Our results suggested that that mRNA levels of CDCA1/2/4/7 related to the prognosis and can be used as a new useful biomarker for predicting the survival of HNSCC patients. The top 5 CDCAs neighboring gene alterations in HNSCCs were found in MYC, STAG1, RAD21, KLHL9 and NDC80. Multivariable Cox proportional hazard model also showed that CD8+ T cells were higher (P<0.05) in HNSCC-HPV-pos patients and that this was related to CDCA1/2/3/4/5/7. This study utilizes online tools to conduct specific gene analyses from free open databases, but our study requires more large-scale genomics research and basic research.
头颈部鳞状细胞癌（HNSCC）是一种包括口腔、嘴唇、喉、口咽和鼻咽等部位的肿瘤。细胞分裂周期相关（CDCA）蛋白家族（CDCA1-8）对正常细胞功能和癌细胞增殖至关重要。我们详细研究了 HNSCC 中各种 CDCAs 的突变特征和表达水平。基于 HNSCC 患者的拷贝数和基因表达数据，我们建立了一个全面的生物信息学分析管道，以深入了解 CDCAs 调节的可能功能和独特预后。我们比较了 HNSCC 中 CDCAs 的转录表达，发现多个数据集的 HNSCC 组织中 CDCA1-8 的 mRNA 表达显著升高。我们还发现 CDCA5/6/8 在 HNSCC 患者中无论是转录还是翻译都过度表达。我们的研究结果表明，CDCA1/2/4/7 的 mRNA 水平与预后相关，可作为预测 HNSCC 患者生存的新的有用生物标志物。在 HNSCC 中发现了前 5 位与 CDCAs 相邻的基因改变，分别为 MYC、STAG1、RAD21、KLHL9 和 NDC80。多变量 Cox 比例风险模型还显示，HPV 阳性的 HNSCC 患者中 CD8+T 细胞更高（P<0.05），这与 CDCA1/2/3/4/5/7 有关。本研究利用在线工具从免费开放数据库中进行特定基因分析，但我们的研究需要更多大规模的基因组学研究和基础研究。
