人源瞬时受体电位通道 A1（TRPA1）是一种固有机械敏感性双分子层门控离子通道。
Human TRPA1 is an inherently mechanosensitive bilayer-gated ion channel.
机构信息
Wallenberg Centre for Molecular Medicine, Linköping University, SE-581 83, Linköping, Sweden; Department of Biomedical and Clinical Sciences (BKV), Faculty of Health Sciences, Linköping University, SE-581 83, Linköping, Sweden.
Department of Clinical Sciences Malmö, Lund University, SE-214 28, Malmö, Sweden.
出版信息
Cell Calcium. 2020 Nov;91:102255. doi: 10.1016/j.ceca.2020.102255. Epub 2020 Jul 18.
The role of mammalian Transient Receptor Potential Ankyrin 1 (TRPA1) as a mechanosensor is controversial. Here, we report that purified human TRPA1 (hTRPA1) with and without its N-terminal ankyrin repeat domain responded with pressure-dependent single-channel current activity when reconstituted into artificial lipid bilayers. The hTRPA1 activity was abolished by the thiol reducing agent TCEP. Thus, depending on its redox state, hTRPA1 is an inherent mechanosensitive ion channel gated by force-from-lipids.
哺乳动物瞬时受体电位锚蛋白 1（TRPA1）作为机械感受器的作用存在争议。在这里，我们报告说，当重新构建到人工脂质双层中时，带有和不带有其 N 端锚蛋白重复结构域的纯化人 TRPA1（hTRPA1）会对压力依赖性单通道电流活动作出响应。还原剂 TCEP 会使 hTRPA1 的活性丧失。因此，根据其氧化还原状态，hTRPA1 是一种固有机械敏感性离子通道，由脂质产生的力来开启。
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