Department of Clinical Sciences, Swedish University of Agricultural Sciences, Uppsala, Sweden; Faculty of Veterinary Sciences, Mahasarakham University, Maha Sarakham, Thailand.
Department of Clinical Sciences, Swedish University of Agricultural Sciences, Uppsala, Sweden.
J Reprod Immunol. 2020 Nov;142:103179. doi: 10.1016/j.jri.2020.103179. Epub 2020 Jul 18.
Seminal plasma (SP) regulates immune responses in the female reproductive tract through specific cytokines. It is not known whether SP from high fertility bulls (H) differs from SP from low fertility bulls (L). In this study, the cytokine response of bovine endometrial epithelial cells (bEEC) in culture was investigated after challenge with SP from two bulls of below average (L) or three bulls of above average fertility (H). The bEECs were challenged with 1% or 4% SP from l- or H-fertility bulls (L1, L4, H1, H4, respectively) or 1% or 4% PBS as control (C1, C4) for 72 h. The culture media were analysed for concentrations (pg/million cells) of transforming growth factor beta (TGF-β1, TGF-β2 and TGF-β3) by Luminex, and Interleukin 6 and 8 (IL-6, IL-8) by ELISA. Challenge significantly affected production of TGF-ß1, TGF-ß2 and IL-8 compared to controls and was affected by bull fertility (p < 0.0001), SP concentration (p < 0.0001) and their interaction (p < 0.0001). A higher production of TGF-β1, TGF-β2 and IL-8 (p < 0.0001), and also IL-6 (p < 0.01), resulted from challenge with high doses of SP, being higher for L than H (p < 0.05). For TGF-β3, fertility of bull (p < 0.05). For TGF-B3, fertility of bull (p < 0.05) and the interaction between fertility and concentration of SP were significant (p < 0.01). In conclusion, 4% SP from L bulls stimulated more TGF-β1, TGF-β2, TGF-β3, IL-6 and IL-8 production than SP from H bulls, indicating that stimulation of the endometrium is relevant for fertility. Seminal plasma from high fertility bulls seems to affect cytokine production in utero positively in inseminated cows.
精浆通过特定的细胞因子调节雌性生殖道的免疫反应。目前尚不清楚高生育力公牛（H）的精浆与低生育力公牛（L）的精浆是否不同。在这项研究中，研究了培养的牛子宫内膜上皮细胞（bEEC）在受到低生育力公牛（L）的 1%或 4%精浆（L1、L4）或高生育力公牛（H）的 1%或 4%精浆（H1、H4）或 PBS（1%或 4%，分别为 C1、C4）刺激后的细胞因子反应，持续 72 小时。通过 Luminex 分析培养基中转化生长因子-β（TGF-β1、TGF-β2 和 TGF-β3）的浓度（pg/百万个细胞），通过 ELISA 分析白细胞介素 6 和 8（IL-6、IL-8）的浓度。与对照相比，刺激显著影响 TGF-β1、TGF-β2 和 IL-8 的产生，并且受公牛生育力（p<0.0001）、精浆浓度（p<0.0001）及其相互作用（p<0.0001）的影响。用高剂量的 SP 刺激会导致 TGF-β1、TGF-β2 和 IL-8（p<0.0001）的产生增加，并且也会导致 IL-6（p<0.01）的产生增加，与 H 相比，L 的刺激更高（p<0.05）。对于 TGF-β3，公牛的生育力（p<0.05）和 SP 浓度与生育力之间的相互作用（p<0.01）有显著差异。总之，L 公牛的 4%精浆比 H 公牛的精浆刺激产生更多的 TGF-β1、TGF-β2、TGF-β3、IL-6 和 IL-8，这表明对子宫内膜的刺激与生育力有关。高生育力公牛的精浆似乎对授精奶牛的子宫内细胞因子产生有积极影响。
