Liu Ying-Ying, Liu Xiao-Kang, Cui Xiao-Die, Chen Min, Li Shuai-Hua, He Dan-Dan, Liu Jian-Hua, Yuan Li, Hu Gong-Zheng, Pan Yu-Shan
College of Animal Husbandry and Veterinary Science, Henan Agricultural University, Zhengzhou, China.
Front Microbiol. 2020 Jul 3;11:1523. doi: 10.3389/fmicb.2020.01523. eCollection 2020.
The objective of this study was to explore the genetic and biological features of the (M)-harboring plasmid pTS14 in strain S14 isolated from a chicken fecal sample. Plasmid pTS14 was identified by conjugation, S1-pulsed-field gel electrophoresis (PFGE), Southern hybridization, and plasmid sequencing. The biological characteristics of pTS14 were assessed via stability, growth kinetics, and starvation survival experiments. Strain S14, belonging to ST3007, harbored a 119-kb (M)-bearing IncF2:A1:B1 conjugative plasmid pTS14. The plasmid pTS14 contained a novel transposon Tn with the genetic structure IS---Δ--(M)--Δ-IS, and the resistance genes (B), (D), , , and . In addition, pTS14 was found to be highly stable in the recipient strain J53. The transconjugant TS14 exhibited a higher survival ratio than J53 under permanent starvation-induced stress. The (M)-bearing IncF2 epidemic plasmid lineage may accelerate the dissemination of (M) and other genes by coselection, which could constitute a potentially serious threat to clinical treatment regimens.
本研究的目的是探索从一份鸡粪便样本中分离出的菌株S14中携带（M）的质粒pTS14的遗传和生物学特征。通过接合、S1脉冲场凝胶电泳（PFGE）、Southern杂交和质粒测序鉴定了质粒pTS14。通过稳定性、生长动力学和饥饿存活实验评估了pTS14的生物学特性。属于ST3007的菌株S14携带一个119 kb的携带（M）的IncF2:A1:B1接合质粒pTS14。质粒pTS14包含一个具有遗传结构IS---Δ--(M)--Δ-IS的新型转座子Tn，以及抗性基因（B）、（D）、 、 和 。此外，发现pTS14在受体菌株J53中高度稳定。在长期饥饿诱导的应激条件下，接合子TS14的存活率高于J53。携带（M）的IncF2流行质粒谱系可能通过共选择加速（M）和其他基因的传播，这可能对临床治疗方案构成潜在的严重威胁。
