Drug Quality and Registration (DruQuaR) Group, Faculty of Pharmaceutical Sciences, Ghent University, Ottergemsesteenweg 460, 9000, Ghent, Belgium.
Department of Physical and Biocoordination Chemistry, Faculty of Pharmacy, Medical University of Łódź, Muszyńskiego 1, 90-151, Łódź, Poland.
J Biol Inorg Chem. 2020 Sep;25(6):875-885. doi: 10.1007/s00775-020-01801-3. Epub 2020 Jul 27.
L-ascorbic acid 2-phosphate magnesium (APMg) salt is a vitamin C derivative frequently used as a raw material in cell and tissue therapy. APMg is not only used as a replacement of the unstable ascorbate, but also shows additional cell-biological functionalities. However, its unknown structural characteristics hamper the mechanistic elucidation of its biological role. Therefore, different techniques were applied for APMg structure characterization. Firstly, the stoichiometric composition was characterized by its solvent, ligand and magnesium content. No crystals of APMg could be obtained; however, a single crystal of APNa, the sodium salt of l-ascorbic acid 2-phosphate, was successfully obtained and its crystal structure was elucidated. FT-IR was applied to further clarify the structure of solid APMg. Finally, the structure of APMg in aqueous solution was explored by potentiometric titration as well as FT-IR.
L-抗坏血酸 2-磷酸镁（APMg）盐是一种维生素 C 衍生物，常用于细胞和组织治疗的原料。APMg 不仅可用作不稳定抗坏血酸的替代品，还具有额外的细胞生物学功能。然而，其未知的结构特征阻碍了其生物学作用的机制阐明。因此，应用了不同的技术来表征 APMg 的结构。首先，通过溶剂、配体和镁含量来表征其化学计量组成。未能获得 APMg 的晶体；然而，成功获得了 L-抗坏血酸 2-磷酸的钠盐 APNa 的单晶，并阐明了其晶体结构。傅里叶变换红外光谱（FT-IR）用于进一步澄清 APMg 的结构。最后，通过电位滴定和 FT-IR 研究了水溶液中 APMg 的结构。
