Clinic of Reconstructive Dentistry, Center of Dental Medicine, University of Zurich, Zurich, Switzerland.
Department of Periodontology, Research Institute for Periodontal Regeneration, College of Dentistry,, Yonsei University, Seoul, South Korea.
Clin Oral Investig. 2021 Mar;25(3):1535-1545. doi: 10.1007/s00784-020-03461-8. Epub 2020 Jul 27.
To assess histopathological and histomorphometric outcomes of soft tissue volume augmentation procedures at pontic sites using a volume-stable cross-linked collagen matrix (VCMX) and a non-cross-linked collagen matrix (XCM).
In twelve adult beagle dogs, the mandibular premolars and first molar were hemisected and the mesial root extracted. Soft tissue augmentation was randomly performed using VCMX, XCM, or a sham-operated control. Sacrifice was performed after 4, 8, and 26 weeks. Non-decalcified sections were analyzed for histopathologic and histomorphometric measurements at four different levels below the crest (1.5, 2.5, 3.5, and 5.5 mm).
Group VCMX presented a greater overall amount of soft tissue at all healing time points, more pronounced fibroblast ingrowth, vascularization, and a substantial new collagen deposition. Over time, group XCM demonstrated faster signs of degradation compared with group VCMX. Four weeks after augmentation, group VCMX yielded a higher mean ridge width compared with groups XCM (2.22 mm VCMX, 0.89 mm XCM (at 2.5 mm); 2.05 mm VCMX, 0.80 mm XCM (at 3.5 mm) p < 0.05) and sham (0.59 mm sham (at 1.5 mm); 0.48 mm (at 2.5 mm); 0.44 mm (at 3.5 mm) p < 0.05). After healing periods of 8 and 26 weeks, measurements in group VCMX remained significantly higher compared with group sham both at 8 weeks (levels of 1.5 mm, 2.5 mm and 5.5 mm) and at 26 weeks (levels of 1.5 mm, 3.5 mm and 5.5 mm) (p < 0.05).
The use of a cross-linked collagen matrix resulted in a greater and more stable ridge width over time compared with control groups.
Soft tissue volume augmentation at pontic sites is more effective when using a cross-linked compared with a non-cross-linked collagen matrix.
评估使用体积稳定交联胶原基质（VCMX）和非交联胶原基质（XCM）在桥体部位进行软组织体积增大手术的组织病理学和组织形态计量学结果。
在 12 只成年比格犬中，下颌前磨牙和第一磨牙被半切，近中根被取出。随机使用 VCMX、XCM 或假手术对照进行软组织增大。在 4、8 和 26 周后进行处死。在牙槽嵴以下 4 个不同水平（1.5、2.5、3.5 和 5.5mm）进行非脱钙切片的组织病理学和组织形态计量学分析。
VCMX 组在所有愈合时间点均显示出更大的软组织总体量，更明显的成纤维细胞向内生长、血管生成和大量新胶原沉积。随着时间的推移，XCM 组比 VCMX 组更快地出现降解迹象。在增大后 4 周，VCMX 组的平均牙槽嵴宽度高于 XCM 组（2.22mm VCMX，0.89mm XCM（在 2.5mm）；2.05mm VCMX，0.80mm XCM（在 3.5mm）p<0.05）和假手术组（0.59mm 假手术组（在 1.5mm）；0.48mm（在 2.5mm）；0.44mm（在 3.5mm）p<0.05）。在 8 周和 26 周的愈合期后，VCMX 组的测量值在 8 周（1.5mm、2.5mm 和 5.5mm 水平）和 26 周（1.5mm、3.5mm 和 5.5mm 水平）时均显著高于假手术组（p<0.05）。
与对照组相比，使用交联胶原基质可使牙槽嵴宽度随时间的推移更大且更稳定。
与非交联胶原基质相比，在桥体部位使用交联胶原基质进行软组织体积增大更有效。
