Fisher M, Mullen R J
Department of Anatomy and Cell Biology, University of Virginia Medical Center, Charlottesville 22908.
Neuron. 1988 Apr;1(2):151-7. doi: 10.1016/0896-6273(88)90199-7.
Cerebella, variably deficient in Purkinje cells, were obtained from aggregation chimeras of either Lurcher or Purkinje cell degeneration mutants. These cerebella were used to analyze the expression of glycerol-3-phosphate dehydrogenase (GPDH) in Bergmann glia. Immunocytochemistry showed apparently normal GPDH expression only in Bergmann glia in the immediate vicinity of surviving Purkinje cells. The number of GPDH-positive Bergmann glia cells associated with isolated Purkinje cells was close to that expected, based on measurements in Golgi-stained, normal cerebella of the Bergmann glia cell's domain. The results support the hypothesis that GPDH expression in Bergmann glia cells depends upon their sustained interaction with Purkinje cells, most likely involving direct cell-cell contact.
从Lurcher或浦肯野细胞变性突变体的聚集嵌合体中获得了浦肯野细胞数量不同程度缺失的小脑。这些小脑被用于分析伯格曼胶质细胞中甘油-3-磷酸脱氢酶（GPDH）的表达。免疫细胞化学显示，仅在存活的浦肯野细胞紧邻区域的伯格曼胶质细胞中，GPDH表达明显正常。基于对经高尔基染色的正常小脑中伯格曼胶质细胞区域的测量，与孤立浦肯野细胞相关的GPDH阳性伯格曼胶质细胞数量接近预期。这些结果支持了这样一种假说，即伯格曼胶质细胞中GPDH的表达取决于它们与浦肯野细胞的持续相互作用，很可能涉及直接的细胞间接触。
