Iorizzo Massimo, Testa Bruno, Lombardi Silvia Jane, Ganassi Sonia, Ianiro Mario, Letizia Francesco, Succi Mariantonietta, Tremonte Patrizio, Vergalito Franca, Cozzolino Autilia, Sorrentino Elena, Coppola Raffaele, Petrarca Sonia, Mancini Massimo, De Cristofaro Antonio
Department of Agriculture, Environmental and Food Sciences, University of Molise, 86100 Campobasso, Italy.
Consorzio Nazionale Produttori Apistici CONAPROA, 86100 Campobasso, Italy.
Antibiotics (Basel). 2020 Jul 24;9(8):442. doi: 10.3390/antibiotics9080442.
is the causative agent of American foulbrood (AFB), a severe bacterial disease that affects larvae of honeybees. The present study evaluated, in vitro, antimicrobial activity of sixty-one strains, against ATCC 9545. Five strains (P8, P25, P86, P95 and P100) that showed the greatest antagonism against ATCC 9545were selected for further physiological and biochemical characterizations. In particular, the hydrophobicity, auto-aggregation, exopolysaccharides production, osmotic tolerance, enzymatic activity and carbohydrate assimilation patterns were evaluated. The five selected strains showed suitable physical and biochemical properties for their use as probiotics in the honeybee diet. The selection and availability of new selected bacteria with good functional characteristics and with antagonistic activity against opens up interesting perspectives for new biocontrol strategies of diseases such as AFB.
是美洲幼虫腐臭病（AFB）的病原体，AFB是一种影响蜜蜂幼虫的严重细菌性疾病。本研究在体外评估了61株菌株对ATCC 9545的抗菌活性。选择了对ATCC 9545表现出最大拮抗作用的5株菌株（P8、P25、P86、P95和P100）进行进一步的生理和生化特性分析。特别评估了其疏水性、自聚集性、胞外多糖产生、渗透压耐受性、酶活性和碳水化合物同化模式。所选的5株菌株表现出适合用作蜜蜂饲料益生菌的物理和生化特性。选择并获得具有良好功能特性且对[病原体名称缺失]具有拮抗活性的新细菌，为AFB等疾病的新生物防治策略开辟了有趣的前景。
