Alippi Adriana M, Reynaldi Francisco J
Centro de Investigaciones de Fitopatología, Facultad de Ciencias Agrarias y Forestales, Universidad Nacional de La Plata, 60 y 118, cc 31, 1900 La Plata, Argentina.
J Invertebr Pathol. 2006 Mar;91(3):141-6. doi: 10.1016/j.jip.2005.12.002. Epub 2006 Feb 3.
The bacterium Paenibacillus larvae, the causative agent of American foulbrood disease of honeybee larvae, occurs throughout the world and is found in many beekeeping areas of Argentina. The potential as biocontrol agents of antagonic aerobic spore-forming bacteria isolated from honey samples and other apiarian sources were evaluated. Each isolate was screened against one strain of Paenibacillus larvae (ATCC 9545) by using a perpendicular streak technique. Ten randomly selected bacterial strains from the group that showed the best antagonistic effect to P. larvae ATCC 9545 were selected for further study. These were identified as Bacillus subtilis (m351), B. pumilus (m350), B. licheniformis (m347), B. cereus (mv33), B. cereus (m387), B. cereus (m6c), B. megaterium (m404), Brevibacillus laterosporus (BLAT169), B. laterosporus (BLAT170), and B. laterosporus (BLAT171). The antagonistic strains were tested against 17 P. larvae strains from different geographical origins by means of a spot test in wells. The analysis of variance and posterior comparison of means by Tukey method (P < 0.01) showed that the best antagonists were B. megaterium (m404), B. licheniformis (m347), B. cereus (m6c), B. cereus (mv33), and B. cereus (m387).
幼虫芽孢杆菌是蜜蜂幼虫美洲幼虫腐臭病的病原体，在世界各地均有出现，在阿根廷的许多养蜂地区也能找到。对从蜂蜜样本和其他养蜂来源分离出的拮抗需氧芽孢杆菌作为生物防治剂的潜力进行了评估。通过垂直划线技术，针对一株幼虫芽孢杆菌（ATCC 9545）对每个分离株进行筛选。从对幼虫芽孢杆菌ATCC 9545表现出最佳拮抗作用的组中随机选择10株细菌菌株进行进一步研究。这些菌株被鉴定为枯草芽孢杆菌（m351）、短小芽孢杆菌（m350）、地衣芽孢杆菌（m347）、蜡样芽孢杆菌（mv33）、蜡样芽孢杆菌（m387）、蜡样芽孢杆菌（m6c）、巨大芽孢杆菌（m404）、侧孢短芽孢杆菌（BLAT169）、侧孢芽孢杆菌（BLAT170）和侧孢芽孢杆菌（BLAT171）。通过孔点滴试验，对这些拮抗菌株针对来自不同地理来源的17株幼虫芽孢杆菌菌株进行了测试。方差分析和采用Tukey法进行的均值事后比较（P < 0.01）表明，最佳拮抗菌株是巨大芽孢杆菌（m404）、地衣芽孢杆菌（m347）、蜡样芽孢杆菌（m6c）、蜡样芽孢杆菌（mv33）和蜡样芽孢杆菌（m387）。
