School of Food Science and Technology, Faculty of Science and Technology, Thammasat University (Rangsit Campus) , Khong Luang, Pathumthani, Thailand.
Functional Ingredient and Food Innovation Research Group, National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA) , Khong Luang, Pathumthani, Thailand.
Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 2020 Sep;37(9):1579-1589. doi: 10.1080/19440049.2020.1778182. Epub 2020 Jul 28.
Aflatoxin B is a naturally occurring mycotoxin that is produced as secondary metabolite by spp., especially and . This is the most severe toxin due to its carcinogenic, mutagenic, and teratogenic properties. Hence, methods for toxin degradation have been received increasing interest from both scientific communities and industries. In this study, 32 isolates of spp. from various fermented cereal products were screened for their aflatoxin B degradation ability. The results indicated the extracellular fraction of BCC 42005 isolated from Iru (African locust bean) potentially possessed aflatoxin B-degrading ability. The maximum activity of the active fraction was at 50°C and pH 8.0. The activity was stable in a wide range of pH (5.0-8.0) and temperature (25-60°C). The aflatoxin B-degrading mechanisms of this strain may be possibly involved by enzyme(s). This extracellular fraction was not toxic at IC 4 mg/ml and it can be combined with water as a soaking agent for maize, which results in 54% of aflatoxin B reduction after contact time 120 min. Hence, the extracellular fraction of BCC 42005 can be further applied as an effective soaking agent in a pretreatment process with a practical and easy-to-implement condition and also probably used to reduce the aflatoxin B contamination in other foods and feeds commodities.
黄曲霉毒素 B 是一种天然存在的真菌毒素，由 spp.，特别是 和 产生的次生代谢物。由于其致癌、致突变和致畸性，这是最严重的毒素。因此，毒素降解方法受到科学界和工业界越来越多的关注。在这项研究中，从各种发酵谷物产品中筛选了 32 株 spp.，以研究它们对黄曲霉毒素 B 的降解能力。结果表明，从 Iru（非洲角豆）中分离出的 BCC 42005 的胞外部分可能具有黄曲霉毒素 B 降解能力。活性部分的最大活性在 50°C 和 pH 8.0 时达到。该活性在广泛的 pH（5.0-8.0）和温度（25-60°C）范围内稳定。该菌株的黄曲霉毒素 B 降解机制可能涉及酶。该胞外部分在 IC 4 时没有毒性mg/ml，它可以与水混合作为玉米的浸泡剂，在接触时间 120 分钟后，黄曲霉毒素 B 的含量降低了 54%。因此，BCC 42005 的胞外部分可以进一步用作预处理过程中的有效浸泡剂，具有实际和易于实施的条件，也可能用于降低其他食品和饲料商品中的黄曲霉毒素 B 污染。
