Li Ting, Chang Xiaoxi, Qiao Zixuan, Ren Guangxi, Zhou Na, Chen Jiaxin, Jiang Dan, Liu Chunsheng
School of Chinese Medicine, Beijing University of Chinese Medicine, Beijing, China.
Shaanxi University of Chinese Medicine, Co-Construct Collaborat Innovat Ctr Chinese Medicine Research, Xianyang, China.
Front Microbiol. 2024 Aug 7;15:1407270. doi: 10.3389/fmicb.2024.1407270. eCollection 2024.
Coix seed is a good product for both medicinal and food use, which is highly susceptible to aflatoxin B (AFB) contamination during field transport, storage, and processing. The aim of this study is to find microbial strains that can solve the problem of contamination of coix seed. In this study, the AFB-degrading microorganism SX1-1 was isolated and identified as a based on morphology, microscopy, and 16S rDNA sequencing. The optimum culture conditions for SX1-1 to degrade AFB were determined to be 12 h. The optimum degradation conditions were 72 h, 57°C, and an initial pH of 8.0. The highest degradation of AFB was observed in the fermentation supernatant of the SX1-1 strain, with a degradation rate of 97.45%. In addition, whole-genome sequencing analysis of this strain revealed the presence of a number of enzymes that could potentially degrade AFB. Importantly, SX1-1 was able to degrade AFB-contaminated coix seed by 50.06% after co-culture. In conclusion, this strain had a high AFB degradation ability, and has great potential and great application as a biocontrol agent for AFB degradation of coix seed.
薏苡仁是一种药食两用的佳品，在田间运输、储存和加工过程中极易受到黄曲霉毒素B（AFB）污染。本研究旨在寻找能够解决薏苡仁污染问题的微生物菌株。在本研究中，通过形态学、显微镜观察和16S rDNA测序分离并鉴定出AFB降解微生物SX1-1为[具体菌种未给出]。确定SX1-1降解AFB的最佳培养条件为12小时。最佳降解条件为72小时、57°C和初始pH值8.0。在SX1-1菌株的发酵上清液中观察到AFB的最高降解率，为97.45%。此外，对该菌株的全基因组测序分析表明存在多种可能降解AFB的酶。重要的是，共培养后SX1-1能够将受AFB污染的薏苡仁降解50.06%。总之，该菌株具有较高的AFB降解能力，作为薏苡仁AFB降解的生物防治剂具有巨大潜力和广阔应用前景。
