Bjørndal Bodil, Aloysius Thomas A, Lund Anders, Slizyte Rasa, Bohov Pavol, Carvajal Ana Karina, Berge Rolf K
Department of Clinical Science University of Bergen Bergen Norway.
SINTEF Ocean Trondheim Norway.
Food Sci Nutr. 2019 Dec 13;8(7):3052-3060. doi: 10.1002/fsn3.1300. eCollection 2020 Jul.
Chicken protein hydrolysates (CPHs) generated from rest raw materials through enzymatic hydrolysis using Corolase PP or Alcalase were shown to reduce inflammation and stimulate hepatic mitochondrial fatty acid oxidation in high-fat-fed mice. This study investigates the effect of CPH diets in atherosclerosis-prone apolipoprotein E-deficient (Apoe) mice. Apoe mice were divided into three groups of 12 animals and fed high-fat diets with casein (control), Alcalase CPH, or Corolase PP CPH. After 12 weeks, mice were sacrificed, blood samples were collected, and aorta was dissected for subsequent analysis. Mice fed Corolase PP CPH but not Alcalase CPH had significantly lower % atherosclerotic plaque area in the aortic arch compared to controls ( = .015 and = .077, respectively). Plasma and liver cholesterol and triacylglycerol remained constant, but levels of the fatty acid C20:5n-3 were increased, accompanied by an elevated delta-5 desaturase index in both CPHs groups. Moreover, a significant reduction of plasma MCP-1 was detected in Corolase PP CPH compared to control. Overall, our data show that protein hydrolysates from chicken reduced atherosclerosis and attenuated systemic risk factors related to atherosclerotic disorders, not related to changes in the level of plasma cholesterol.
通过使用嗜热菌蛋白酶PP或碱性蛋白酶对剩余原料进行酶解产生的鸡肉蛋白水解物（CPH），已证明可减轻高脂喂养小鼠的炎症并刺激肝脏线粒体脂肪酸氧化。本研究调查了CPH饮食对易患动脉粥样硬化的载脂蛋白E缺陷（Apoe）小鼠的影响。将Apoe小鼠分为三组，每组12只，分别用酪蛋白（对照）、碱性蛋白酶CPH或嗜热菌蛋白酶PP CPH喂养高脂饮食。12周后，处死小鼠，采集血样，并解剖主动脉进行后续分析。与对照组相比，喂食嗜热菌蛋白酶PP CPH而非碱性蛋白酶CPH的小鼠主动脉弓中的动脉粥样硬化斑块面积百分比显著降低（分别为=0.015和=0.077）。血浆和肝脏中的胆固醇和三酰甘油保持不变，但脂肪酸C20:5n-3的水平升高，同时两个CPH组的δ-5去饱和酶指数均升高。此外，与对照组相比，在嗜热菌蛋白酶PP CPH中检测到血浆MCP-1显著降低。总体而言，我们的数据表明，鸡肉蛋白水解物可减轻动脉粥样硬化，并减轻与动脉粥样硬化疾病相关的全身风险因素，这与血浆胆固醇水平的变化无关。
