Zhu CuiFeng, Zhang Wei, Mu Bo, Zhang Fan, Lai NanNan, Zhou JianXin, Xu AiMin, Liu JianGuo, Li Yong
Department of Nutrition, ShenZhen Hospital of Southern Medical University, Shenzhen City, Guangdong Province People's Republic of China.
Department of Nutrition, Shenzhen HengSheng Affiliated Hospital, Southern Medical University, Shenzhen City, Guangdong Province People's Republic of China.
J Food Sci Technol. 2017 Jul;54(8):2260-2269. doi: 10.1007/s13197-017-2663-z. Epub 2017 Jun 14.
The present study was conducted to investigate the effects of marine collagen peptides (MCPs) on glucose metabolism and insulin resistance using a rat model of type 2 diabetes mellitus (T2DM). Forty T2DM obese Wistar rats were randomly assigned to receive varying doses of MCPs or a vehicle control for 4 weeks. Blood glucose and insulin levels, as well as oxidative stress and inflammation were measured. The expression of glucose transporter type 4 (GLUT4) in skeletal muscles and peroxisome proliferator-activated receptor-α (PPAR-α) in livers of T2DM rats was also measured. It was found that in the group of 9.0 g/kg/day MCPs significantly improved glucose, insulin, and homeostatic model assessment-insulin resistance, and increased the insulin sensitivity index (ISI). In addition, the groups of 4.5 and 2.25 g/kg/day MCPs significantly improved liver steatosis. It was also found that MCPs decreased expression of oxidative stress biomarkers and inflammatory cytokines and adipocytokines in T2DM rats. In conclusion, medium and high doses of MCPs (≥4.5 g/kg/day) improved glucose metabolism and insulin sensitivity in T2DM rats. These beneficial effects of MCPs may be mediated by decreasing oxidative stress and inflammation and by up-regulating GLUT4, and PPAR-α activity.
本研究旨在使用2型糖尿病（T2DM）大鼠模型，研究海洋胶原蛋白肽（MCPs）对葡萄糖代谢和胰岛素抵抗的影响。40只T2DM肥胖Wistar大鼠被随机分配接受不同剂量的MCPs或赋形剂对照，为期4周。测量血糖和胰岛素水平，以及氧化应激和炎症指标。还测量了T2DM大鼠骨骼肌中葡萄糖转运蛋白4（GLUT4）的表达和肝脏中过氧化物酶体增殖物激活受体-α（PPAR-α）的表达。结果发现，9.0 g/kg/天MCPs组显著改善了血糖、胰岛素和稳态模型评估-胰岛素抵抗，并提高了胰岛素敏感性指数（ISI）。此外，4.5和2.25 g/kg/天MCPs组显著改善了肝脏脂肪变性。还发现MCPs降低了T2DM大鼠氧化应激生物标志物、炎性细胞因子和脂肪细胞因子的表达。总之，中高剂量的MCPs（≥4.5 g/kg/天）改善了T2DM大鼠的葡萄糖代谢和胰岛素敏感性。MCPs的这些有益作用可能是通过降低氧化应激和炎症以及上调GLUT4和PPAR-α活性来介导的。
