Anti- activity and chemical compositions of aquatic extract of L. and L.: An in vitro study.

This study aimed to determine the chemical compositions of crude aquatic extracts of L. and L., and their anti- activity. Crude aquatic extraction of aerial parts of L. and L. was performed. GC-MS and HTPLC analyses were carried out. MTT assay was performed on Vero cells treated by different concentrations (Log from 10 to 10) of the extracts. The anti- activity of the concentrations was investigated using vital staining. Menthol (99.23%) and limonene (0.227%) were the major compounds of the aquatic extract of L. Phytochemical compositions of L. were terpenoids, esterols, and flavonoids. The cell toxicity of L. was lower than L. (CC50 > 10 versus. ≥ 10). Aquatic extract of L. showed higher anti- activity (LC50 ≥ 10) than L. (LC50 ≥ 10). Statistically significant cell toxicity and anti- activity ( < .05) were seen regarding the different concentrations of L. and L. Both L. and L. revealed anti- activities. Cell toxicity of L. was significantly higher than L. L. extract could be more applicable due to its lower cell toxicity.