Department of Biology, College of Science, Princess Nourah bint Abdulrahman University, P.O. Box 84428, Riyadh 11671, Saudi Arabia.
Department of Medical Laboratory Science, College of Applied Medical Sciences, University of Ha'il, Ha'il 55476, Saudi Arabia.
Molecules. 2022 Jul 28;27(15):4824. doi: 10.3390/molecules27154824.
Multiple biological functions of extract were evaluated in the current work. Phytochemical components of the extract were detected by Gas Chromatography-Mass Spectrometry (GC-MS) and High-performance liquid chromatography (HPLC). Moreover, extract was estimated for antioxidant potential by 2,2-Diphenyl-1-picryl-hydrazyl-hydrate (DPPH) free radical scavenging, antimicrobial activity by well diffusion, and anticoagulant activity via prothrombin time (PT) and activated partial thromboplastin time (APTT). GC-MS analysis detected compounds including cholesterol margarate, stigmast-5-en-3-ol, 19-nor-4-androstenediol, androstan-17-one, pulegone-1,2-epoxide, isochiapin B, dotriacontane, hexadecanoic acid and neophytadiene. Chrysoeriol (15.36 µg/mL) was followed by kaempferol (11.14 µg/mL) and 7-OH flavone (10.14 µg/mL), catechin (4.11 µg/mL), hisperdin (3.05 µg/mL), and luteolin (2.36 µg/mL) were detected by HPLC as flavonoids, in addition to ferulic (13.19 µg/mL), cinnamic (12.69 µg/mL), caffeic (11.45 µg/mL), pyrogallol (9.36 µg/mL), -coumaric (5.06 µg/mL) and salicylic (4.17 µg/mL) as phenolics. Antioxidant activity was detected with IC 18 µg/mL, hemolysis inhibition was recorded as 79.8% at 1000 μg/mL, and PT and APTT were at 21.5 s and 49.5 s, respectively, at 50 μg/mL of extract. The acute toxicity of extract was recorded against PC3 (IC 97.99 µg/mL) and MCF7 (IC 80.21 µg/mL). Antimicrobial activity of extract was documented against , , , , , but not against black fungus . Molecular docking was applied using MOE (Molecular Operating Environment) to explain the biological activity of neophytadiene, luteolin, chrysoeriol and kaempferol. These compounds could be suitable for the development of novel pharmacological agents for treatment of cancer and bacterial infections.
本研究评价了 提取物的多种生物学功能。采用气相色谱-质谱联用（GC-MS）和高效液相色谱（HPLC）检测 提取物的植物化学成分。此外，通过 2,2-二苯基-1-苦基肼水合物（DPPH）自由基清除法评估 提取物的抗氧化潜力，采用平板扩散法评估其抗菌活性，通过凝血酶原时间（PT）和活化部分凝血活酶时间（APTT）评估其抗凝活性。GC-MS 分析检测到包括胆固醇 margarate、豆甾-5-烯-3-醇、19-去甲-4-雄烯二醇、雄甾烷-17-酮、胡椒酮-1,2-环氧、异香叶兰 B、三十烷、十六烷酸和新 Phytadiene 的化合物。HPLC 检测到的黄酮类化合物包括芹黄素（15.36μg/mL）、山奈酚（11.14μg/mL）和 7-羟基黄酮（10.14μg/mL），儿茶素（4.11μg/mL）、胡椒酸（3.05μg/mL）和木犀草素（2.36μg/mL），此外还有阿魏酸（13.19μg/mL）、肉桂酸（12.69μg/mL）、咖啡酸（11.45μg/mL）、焦性没食子酸（9.36μg/mL）、-对香豆酸（5.06μg/mL）和水杨酸（4.17μg/mL）。抗氧化活性的 IC 为 18μg/mL，在 1000μg/mL 时溶血抑制率为 79.8%，在 50μg/mL 时 PT 和 APTT 分别为 21.5s 和 49.5s。 提取物对 PC3（IC 97.99μg/mL）和 MCF7（IC 80.21μg/mL）的急性毒性记录为无。 提取物对 、 、 、 、 有抗菌活性，但对黑曲霉无活性。采用 MOE（分子操作环境）进行分子对接，以解释新 Phytadiene、木犀草素、芹黄素和山奈酚的生物学活性。这些化合物可能适合开发用于治疗癌症和细菌感染的新型药理学制剂。
