HIV 阳性患者弓形虫感染的血清分子评估。

Sero-molecular evaluation of Toxoplasma gondii infection among HIV-positive patients.

机构信息

Infectious and Tropical Disease Research Center, Tabriz University of Medical Sciences, University Main Street, 51368 Tabriz, Iran.

Department of Parasitology and Mycology, Tabriz University of Medical Sciences, University Main Street, 51368 Tabriz, Iran.

出版信息

Trans R Soc Trop Med Hyg. 2019 Dec 1;113(12):771-775. doi: 10.1093/trstmh/trz082.

DOI:10.1093/trstmh/trz082

PMID:31495900

Abstract

BACKGROUND

Toxoplasmosis is one of the most common comorbidities in HIV-positive patients with CD4+ T lymphocytes below 200 cells/μl. Early diagnosis and treatment of toxoplasmosis reduces the mortality rate in HIV-positive people. The aim of this study was to estimate the seroprevalence of Toxoplasma gondii infection in HIV-positive patients in northwest Iran using serological and molecular methods.

METHODS

This prospective cross-sectional study included 124 HIV-positive outpatients and was conducted from January to May 2016. Anti-T. gondii IgM and IgG antibodies were detected from sera samples by chemiluminescence, while buffy coat samples were analyzed by RT-PCR for DNA detection. Patients' socioepidemiological data were collected.

RESULTS

Using chemiluminescence, 47/124 samples (37.9%) were positive for anti-Toxoplasma IgG antibodies, 2/124 samples (1.62%) were positive for IgM antibodies while 2/124 samples (1.62%) contained both IgM and IgG. There were no IgM-positive or IgG-negative patients. RT-PCR revealed four (3.22%) positive samples. On the basis of the results, a statistically significant relationship was found between anti-Toxoplasma IgG antibody seropositivity and residence (p=0.012).

CONCLUSIONS

The study showed a relatively low seroprevalence of anti-T. gondii IgG and IgM antibodies in HIV-positive patients in northwest Iran, while the prevalence was much higher in other regions of Iran. However, regular screening for T. gondii antibodies and early initiation of therapy are very important to decrease the mortality rate in HIV-positive patients.

摘要

背景

在 CD4+T 淋巴细胞低于 200 个/μl 的 HIV 阳性患者中，弓形体病是最常见的合并症之一。早期诊断和治疗弓形体病可降低 HIV 阳性人群的死亡率。本研究旨在使用血清学和分子方法估计伊朗西北部 HIV 阳性患者中弓形体感染的血清流行率。

方法

这是一项前瞻性的横断面研究，纳入了 124 名 HIV 阳性门诊患者，于 2016 年 1 月至 5 月进行。采用化学发光法检测血清样本中的抗弓形体 IgM 和 IgG 抗体，同时用 RT-PCR 分析血涂片样本以检测 DNA。收集患者的社会人口统计学数据。

结果

使用化学发光法，124 个样本中有 47 个（37.9%）抗弓形体 IgG 抗体阳性，2 个（1.62%）样本 IgM 抗体阳性，2 个（1.62%）样本同时含有 IgM 和 IgG。没有 IgM 阳性或 IgG 阴性的患者。RT-PCR 显示 4 个（3.22%）阳性样本。根据结果，发现抗弓形体 IgG 抗体血清阳性与居住地之间存在统计学显著关系（p=0.012）。