Pakbin Babak, Akhondzadeh Basti Afshin, Khanjari Ali, Azimi Leila, Karimi Abdollah
Department of Food Hygiene and Quality of Control Faculty of Veterinary Medicine University of Tehran Tehran Iran.
Pediatric Infections Research Center Research Institute of children's Health Shahid Beheshti University of Medical Sciences Tehran Iran.
Food Sci Nutr. 2020 May 28;8(7):3665-3672. doi: 10.1002/fsn3.1649. eCollection 2020 Jul.
serotype O157: H7 and type 1 as the Shiga toxin-producing bacteria cause some acute gastrointestinal and extraintestinal diseases such as hemorrhagic uremic syndrome and bloody diarrhea in human. genes are the key virulence factors in these pathogens. The aim of this study was to develop HRMA assay to differentiate gene for detection of serotype O157: H7 and type 1 and determine the prevalence of these pathogens in food samples using this method. PCR-HRMA assay and gold standard methods have been carried out for identification of pathogens among 135 different food samples. We found HRMA method a sensitive and specific assay (100 and 100%, respectively) for differentiation of gene, consequently, detection of these pathogens in food samples. Also, the highest prevalence of serotype O157: H7 and type 1 harboring gene was observed in raw milk and vegetable salad samples, respectively. HRMA as a rapid, inexpensive, sensitive and specific method is suggested to be used for differentiation of gene to detect serotype O157: H7 and type 1 as the key pathogens for safety evaluation of food samples.
O157:H7血清型和1型作为产志贺毒素细菌可导致人类一些急性胃肠道和肠外疾病，如出血性尿毒症综合征和血性腹泻。基因是这些病原体中的关键毒力因子。本研究的目的是开发高分辨率熔解曲线分析（HRMA）方法，以区分用于检测O157:H7血清型和1型的基因，并使用该方法确定这些病原体在食品样本中的流行率。已采用PCR-HRMA分析和金标准方法对135份不同食品样本中的病原体进行鉴定。我们发现HRMA方法对于区分基因是一种灵敏且特异的分析方法（分别为100%和100%），因此可用于检测食品样本中的这些病原体。此外，分别在生牛奶和蔬菜沙拉样本中观察到携带该基因的O157:H7血清型和1型的最高流行率。HRMA作为一种快速、廉价、灵敏且特异的方法，建议用于区分该基因，以检测作为食品样本安全评估关键病原体的O157:H7血清型和1型。
