Department of Pharmacology and Toxicology, University of Benin, Benin City, Edo State, Nigeria.
Department of Physiology, School of Basic Medical Sciences, College of Medical Sciences, University of Benin, Benin City, Nigeria.
Reprod Sci. 2021 Jan;28(1):102-120. doi: 10.1007/s43032-020-00273-9. Epub 2020 Jul 28.
Polycystic ovary syndrome (PCOS) affects up to 10% of women within reproductive ages and has been a cause of infertility and poor quality of life. Alteration in the oxidant-antioxidant profile occurs in PCOS. This study, therefore, investigates the contribution of ascorbic acid (AA) and alpha-tocopherol(ATE) on different PCOS parameters. The mifepristone and letrozole models were used, and young mature female mice were randomly assigned to groups of six per group. On PCOS induction with either mifepristone or letrozole, mice were administered AA and ATE at doses ranging from 10-1000mg/kg to 0.1-1000 mg/kg in the respective models. Vaginal cytology, body weights, and temperature, as well as blood glucose, testosterone, and insulin levels, were measured. Total antioxidant capacity and malondialdehyde levels were analyzed. Determination of gene expression of some reactive oxygen species and histomorphological analysis on the ovaries and uteri were performed. At the end of the experiments, AA and ATE restored reproductive cycling, with AA being more effective. AA and ATE increased fasting blood glucose but had no significant effect on serum insulin levels. AA decreased testosterone levels, but ATE caused slight increases. AA and ATE both increased total antioxidant capacity and decreased malondialdehyde levels. AA and ATE also slightly upregulated the mRNA expressions of catalase, superoxide dismutase, and heme oxygenase 1 mainly. AA and ATE also decreased ovarian weight and mostly resolved cysts in the ovaries and congestion in the uterus. This study has shown that AA and ATE are beneficial in the therapy of PCOS.
多囊卵巢综合征（PCOS）影响了多达 10%的育龄妇女，是导致不孕和生活质量下降的原因之一。在 PCOS 中，氧化还原状态发生改变。因此，本研究调查了抗坏血酸（AA）和α-生育酚（ATE）对不同 PCOS 参数的贡献。使用米非司酮和来曲唑模型，将年轻成熟的雌性小鼠随机分为每组六只的六组。在用米非司酮或来曲唑诱导 PCOS 时，分别在各模型中以 10-1000mg/kg 至 0.1-1000mg/kg 的剂量给予 AA 和 ATE。测量阴道细胞学、体重和体温以及血糖、睾酮和胰岛素水平。分析总抗氧化能力和丙二醛水平。测定一些活性氧的基因表达，并对卵巢和子宫进行组织形态学分析。在实验结束时，AA 和 ATE 恢复了生殖周期，AA 更有效。AA 和 ATE 增加空腹血糖，但对血清胰岛素水平没有显著影响。AA 降低了睾酮水平，但 ATE 导致轻微升高。AA 和 ATE 均增加了总抗氧化能力并降低了丙二醛水平。AA 和 ATE 还略微上调了过氧化氢酶、超氧化物歧化酶和血红素加氧酶 1 的 mRNA 表达。AA 和 ATE 还降低了卵巢重量，并主要解决了卵巢中的囊肿和子宫充血。本研究表明，AA 和 ATE 对 PCOS 的治疗有益。
