抗氧化活性的体内和体外评价方法综述。
Review on in vivo and in vitro methods evaluation of antioxidant activity.
机构信息
Department of Pharmacy, Jahangirnagar University, Savar, Dhaka-1342, Bangladesh.
出版信息
Saudi Pharm J. 2013 Apr;21(2):143-52. doi: 10.1016/j.jsps.2012.05.002. Epub 2012 Jun 15.
Abstract
A good number of abstracts and research articles (in total 74) published, so far, for evaluating antioxidant activity of various samples of research interest were gone through where 407 methods were come across, which were repeated from 29 different methods. These were classified as in vitro and in vivo methods. And those are described and discussed below in this review article. In the later part of this review article, frequency of in vitro as well as in vivo methods is analyzed with a bar diagram. Solvents are important for extracting antioxidants from natural sources. Frequency of solvents used for extraction is also portrayed and the results are discussed in this article. As per this review there are 19 in vitro methods and 10 in vivo methods that are being used for the evaluation of antioxidant activity of the sample of interest. DPPH method was found to be used mostly for the in vitro antioxidant activity evaluation purpose while LPO was found as mostly used in vivo antioxidant assay. Ethanol was with the highest frequency as solvent for extraction purpose.
摘要
迄今为止，已经发表了大量的摘要和研究文章（共计 74 篇），用于评估各种研究样本的抗氧化活性，其中涉及了 407 种方法，这些方法是从 29 种不同的方法中重复出现的。这些方法被分为体外和体内方法。在本文的后续部分，将通过条形图分析体外和体内方法的频率。溶剂对于从天然来源中提取抗氧化剂非常重要。本文还描述和讨论了用于提取的溶剂的频率及其结果。根据本综述，有 19 种体外方法和 10 种体内方法用于评估感兴趣样本的抗氧化活性。发现 DPPH 方法主要用于体外抗氧化活性评估，而 LPO 则主要用于体内抗氧化测定。乙醇是最常用的提取溶剂。
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