Kim Sang-Mo, Markkandan Kesavan, Lee Jong-Young, Kim Gye-Woong, Yoo Jae Young
Department of Animal Resources Science, College of Industrial Sciences, Kongju National University, Yesan-eup, Yesan-gun, Chungcheongnam-do 32588, Korea.
Oneomics Co Ltd., Bucheon-si, Gyeonggi-do 14585, Korea.
Animals (Basel). 2020 Jul 27;10(8):1279. doi: 10.3390/ani10081279.
Carcass quality traits, such as lean depth and loin depth, are of extreme economic importance for the swine industry. This study aimed to identify the gene expression pattern related to carcass quality in crossbred pigs ((Landrace × Yorkshire) × Duroc). In total, 20 crossbred pigs were used in this study and they were divided into two groups (class I grade, n = 10; class II grade, n = 10) based on the carcass grades. Total RNA samples extracted from the loin muscles of both groups were submitted for RNA-seq. The quality assessment of the sequencing reads resulted in 25,458 unigenes and found 12,795 candidate coding unigenes with homology to other species after annotation. Differentially expressed gene (DEG) analysis of the two groups revealed 282 up-regulated and 189 down-regulated genes ( ≤ 0.01), linked to tissue development, striated muscle tissue development, tissue morphogenesis, and lipid metabolic process gene ontology (GO) terms. Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis highlighted genes related to the calcium signaling pathway, melanogenesis, the sphingolipid signaling pathway, the apelin signaling pathway, and the mTOR signaling pathway. We constructed an expressed gene profile, which may serve as a resource for genomic studies focused on uncovering the molecular mechanisms underlying carcass quality in crossbred pigs.
胴体品质性状，如瘦肉深度和腰部深度，对养猪业具有极其重要的经济意义。本研究旨在确定杂交猪（长白猪×大白猪）×杜洛克猪中与胴体品质相关的基因表达模式。本研究共使用了20头杂交猪，并根据胴体等级将它们分为两组（I级，n = 10；II级，n = 10）。从两组猪的腰部肌肉中提取的总RNA样本被送去进行RNA测序。对测序读数的质量评估产生了25458个单基因，并在注释后发现了12795个与其他物种具有同源性的候选编码单基因。两组的差异表达基因（DEG）分析揭示了282个上调基因和189个下调基因（≤0.01），这些基因与组织发育、横纹肌组织发育、组织形态发生以及脂质代谢过程基因本体（GO）术语相关。京都基因与基因组百科全书（KEGG）富集分析突出了与钙信号通路、黑色素生成、鞘脂信号通路、apelin信号通路和mTOR信号通路相关的基因。我们构建了一个表达基因图谱，它可以作为基因组研究的资源，专注于揭示杂交猪胴体品质背后的分子机制。
