Saini B L, Gaur G K, Sahoo N R, Mendiratta S K, Kumar A, Naha B C, Baranwal A, Yadav V, Jaiswal R K
Division of Animal Genetics, ICAR-IVRI, Izatnagar, 243122, India.
Livestock Production and Management Section, ICAR-IVRI, Izatnagar, 243122, India.
Mol Biol Rep. 2018 Dec;45(6):1575-1585. doi: 10.1007/s11033-018-4263-z. Epub 2018 Oct 4.
This study was designed to screen the crossbred pigs for SNPs in five candidate genes, associated with pork quality traits and to differentiate their genotypes by PCR-RFLP. The results indicated that genotypes of crossbred pigs were NN (90%) and Nn (10%) for RYR1; RR (83%) and QR (17%) for PRKAG3; HH (98%), Hh (1%) and hh (1%) for HFABP; DD (99%) and CD (1%) for MYF-5; and AG (57%), GG (26%) and AA (17%) for MC4R SNPs, respectively. Allelic frequencies for five SNPs {RYR1 (1843C>T), PRKAG3 (c.599G>A), HFABP (c.1322C>T), MYF-5 (c.1205A>C) and MC4R (c.1426A>G)} were 0.95 and 0.05 (N/n), 0.08 and 0.92 (Q/R), 0.99 and 0.01 (H/h), 0.00 and 1.00 (C/D) and 0.45 and 0.55 (A/G), respectively. The effect of RYR1 (1843C>T) SNP was significant on pH (P < 0.05), pH (P < 0.05) and protein % (P < 0.05). The PRKAG3 (c.599G>A) and MC4R (c.1426A>G) SNP had significant association with dressing percentages. The results revealed that RYR1, PRKAG3 and MC4R SNPs may be used in marker associated selection for pork quality traits in crossbred pigs.
本研究旨在筛选与猪肉品质性状相关的五个候选基因中的单核苷酸多态性（SNP），并通过聚合酶链反应-限制性片段长度多态性（PCR-RFLP）对杂交猪的基因型进行区分。结果表明，杂交猪RYR1基因的基因型为NN（90%）和Nn（10%）；PRKAG3基因的基因型为RR（83%）和QR（17%）；HFABP基因的基因型为HH（98%）、Hh（1%）和hh（1%）；MYF-5基因的基因型为DD（99%）和CD（1%）；MC4R基因SNP的基因型为AG（57%）、GG（26%）和AA（17%）。五个SNP{RYR1（1843C>T）、PRKAG3（c.599G>A）、HFABP（c.1322C>T）、MYF-5（c.1205A>C）和MC4R（c.1426A>G）}的等位基因频率分别为0.95和0.05（N/n）、0.08和0.92（Q/R）、0.99和0.01（H/h）、0.00和1.00（C/D）以及0.45和0.55（A/G）。RYR1（1843C>T）SNP对pH值（P < 0.05）、pH值（P < 0.05）和蛋白质百分比（P < 0.05）有显著影响。PRKAG3（c.599G>A）和MC4R（c.1426A>G）SNP与屠宰率有显著关联。结果表明，RYR1、PRKAG3和MC4R基因的SNP可用于杂交猪猪肉品质性状的标记辅助选择。
