过表达全羧化酶合成酶预测乳腺癌淋巴结转移和不良预后。
Overexpression of Holocarboxylase Synthetase Predicts Lymph Node Metastasis and Unfavorable Prognosis in Breast Cancer.
机构信息
Department of Biochemistry, Faculty of Science, Mahidol University, Bangkok, Thailand.
Brighton College, Brighton, U.K.
出版信息
Anticancer Res. 2020 Aug;40(8):4557-4565. doi: 10.21873/anticanres.14461.
BACKGROUND/AIM: Holocarboxylase synthetase (HLCS) catalyzes the specific attachment of biotin onto biotin-dependent carboxylases (BDCs) which play important roles in intermediary metabolism. Previous studies show that BDCs are overexpressed in many cancer types. However, expression of HLCS in cancerous tissues has not been reported.
MATERIALS AND METHODS
Immunohistochemistry was used to investigate HLCS expression in breast tissue obtained from 65 Thai patients, and the correlation between its expression and key clinical-pathological parameters was assessed. The role of HLCS in supporting invasion was investigated in HLCS-knockdown MCF-7 cells.
RESULTS
Overexpression of HLCS was significantly associated with metastasis of breast cancer cells to other lymph nodes but not the sentinel and axillary lymph nodes - a finding supported in cellular invasion assays using HLCS knockdown cells. Furthermore, overexpression of HLCS reduced survival time of patients with breast cancer.
CONCLUSION
HLCS appears to be a prognostic marker for patients with breast cancer.
背景/目的： 丙酰基辅酶 A 羧化酶合成酶 (HLCS) 催化生物素特异性地附着于生物素依赖性羧化酶 (BDC) 上，BDC 在中间代谢中发挥重要作用。先前的研究表明，BDC 在许多癌症类型中过度表达。然而，在癌组织中 HLCS 的表达尚未报道。
材料和方法
使用免疫组织化学方法检测 65 例泰国患者乳腺组织中 HLCS 的表达，并评估其表达与关键临床病理参数之间的相关性。在 HLCS 敲低 MCF-7 细胞中研究 HLCS 在支持侵袭中的作用。
结果
HLCS 的过表达与乳腺癌细胞向其他淋巴结转移显著相关，但与前哨淋巴结和腋窝淋巴结转移无关——这一发现得到了使用 HLCS 敲低细胞进行细胞侵袭实验的支持。此外，HLCS 的过表达降低了乳腺癌患者的生存时间。
结论
HLCS 似乎是乳腺癌患者的预后标志物。
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