各种刺激剂在小鼠脾细胞和骨髓细胞中诱导的鲁米诺增强化学发光的动力学。
Kinetics of luminol-enhanced chemiluminescence induced in murine splenocytes and bone marrow cells by various stimulating agents.
作者信息
Cíz M, Lojek A
机构信息
Institute of Biophysics, Czechoslovak Academy of Sciences, Brno.
出版信息
Folia Biol (Praha). 1993;39(2):106-16.
The luminol-amplified chemiluminescence (CL) activity of murine splenocytes and bone marrow cells was investigated and compared. Phagocytosis was activated by adding starch grains, opsonized zymosan particles (OZP), or phorbol myristate acetate (PMA) to cell suspensions. CL was studied within a concentration range of 3.13 x 10(6)-1.25 x 10(8) splenocytes/ml, and 1.56 x 10(6)-6.25 x 10(7) bone marrow cells/ml. The CL reaction intensity was increasing with rising cell concentration throughout the whole range studied. Starch grains and OZP elicited a unimodal kinetics of the CL response with a single peak after 10 min for splenocytes and 13-16 min for bone marrow cells. PMA activation induced a bimodal reaction with the first peak appearing after 3 min for either of the cell populations, and the second maximal peak being recorded after 6-7 min for splenocytes and 9-10 min for bone marrow cells.
对小鼠脾细胞和骨髓细胞的鲁米诺增强化学发光（CL）活性进行了研究和比较。通过向细胞悬液中添加淀粉颗粒、调理酵母聚糖颗粒（OZP）或佛波酯（PMA）来激活吞噬作用。在3.13×10⁶ - 1.25×10⁸个脾细胞/毫升和1.56×10⁶ - 6.25×10⁷个骨髓细胞/毫升的浓度范围内研究CL。在所研究的整个浓度范围内，CL反应强度随细胞浓度的升高而增加。淀粉颗粒和OZP引发了CL反应的单峰动力学，脾细胞在10分钟后出现单峰，骨髓细胞在13 - 16分钟后出现单峰。PMA激活诱导了双峰反应，两种细胞群体在3分钟后均出现第一个峰，脾细胞在6 - 7分钟后记录到第二个最大峰，骨髓细胞在9 - 10分钟后记录到第二个最大峰。
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