Mina and Everard Goodman Faculty of Life Sciences, Bar-Ilan University, Ramat-Gan, Israel.
Methods Mol Biol. 2021;2181:213-227. doi: 10.1007/978-1-0716-0787-9_13.
Following A-to-I editing of double-stranded RNA (dsRNA) molecules, sequencing reactions interpret the edited inosine (I) as guanosine (G). For this reason, current methods to detect A-to-I editing sites work to align RNA sequences to their reference DNA sequence in order to reveal A-to-G mismatches. However, areas with heavily edited reads produce dense clusters of A-to-G mismatches that hinder alignment, and complicate correct identification of the sites. The presented approach employs prudent alignment and examination of excessive mismatch events, enabling high-accuracy detection of hyper-edited reads and sites.
双链 RNA（dsRNA）分子发生 A 到 I 的编辑后，测序反应将编辑后的次黄嘌呤（I）解释为鸟嘌呤（G）。出于这个原因，目前检测 A 到 I 编辑位点的方法是将 RNA 序列与参考 DNA 序列进行比对，以揭示 A 到 G 的错配。然而，编辑严重的区域会产生密集的 A 到 G 错配簇，从而阻碍比对，并使正确识别编辑位点变得复杂。所提出的方法采用谨慎的比对和对过度错配事件的检查，从而能够实现对超编辑读取和位点的高精度检测。
