Li Bowei, Qi Ji, Fu Longwen, Han Jinglong, Choo Jaebum, deMello Andrew J, Lin Bingcheng, Chen Lingxin
CAS Key Laboratory of Coastal Environmental Processes and Ecological Remediation, Shandong Key Laboratory of Coastal Environmental Processes, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai, 264003, China.
School of Environment and Materials Engineering and College of Chemistry and Chemical Engineering, Yantai University, Yantai, 264005, China.
Biosens Bioelectron. 2020 Oct 1;165:112282. doi: 10.1016/j.bios.2020.112282. Epub 2020 Jun 27.
To date, 55% of inhabitants of the developing world still live in rural regions and they have a very urgent need to improve the level of disease diagnosis, especially in resource-limited settings. Herein, we present a microfluidic system that centrifuges whole blood and quantifies contained biomarkers in a rapid, feasible and integrated way. Such a device provides a blood-in/answer-out capability and is small enough to be carried by any individual in any environment. The successful integration a hand-powered centrifuge and immunoassay unit within a rotational paper-based device allows for diagnostic application by untrained users and in environments where access to electricity cannot be assumed. In addition, the low cost (~$ 0.5), light weight and small instrumental footprint make the device ideally suited for rapid on-site detection. To validate the applicability of the system in a clinical diagnostic testing, we successfully perform enzyme-linked immunosorbent assay (ELISA) analysis of carcinoembryonic antigen and alpha fetoprotein from human blood samples. We expect that this powerful platform technology will provide the opportunities for point-of-care diagnosis in resource-limited settings.
迄今为止，发展中世界55%的居民仍生活在农村地区，他们迫切需要提高疾病诊断水平，尤其是在资源有限的环境中。在此，我们展示了一种微流控系统，该系统可对全血进行离心，并以快速、可行且集成的方式对所含生物标志物进行定量。这样的设备具备“血进/结果出”的能力，体积小到可由任何人在任何环境中携带。在基于旋转纸的设备中成功集成手动离心机和免疫分析单元，使得未经培训的用户在无法保证供电的环境中也能进行诊断应用。此外，低成本（约0.5美元）、轻重量和小仪器占地面积使该设备非常适合快速现场检测。为验证该系统在临床诊断测试中的适用性，我们成功地对人血样本中的癌胚抗原和甲胎蛋白进行了酶联免疫吸附测定（ELISA）分析。我们期望这种强大的平台技术将为资源有限环境中的即时诊断提供机会。
