Abate Mahlet Fasil, Ahmed Metages Gashaw, Li Xingrui, Yang Chaoyong, Zhu Zhi
The MOE Key Laboratory of Spectrochemical Analysis & Instrumentation, The Key Laboratory of Chemical Biology of Fujian Province, State Key Laboratory of Physical Chemistry of Solid Surfaces, Collaborative Innovation Center of Chemistry for Energy Materials, Department of Chemical Biology, College of Chemistry and Chemical Engineering, Pen-Tung Sah Institute of Micro-Nano Science and Technology, Xiamen University, Xiamen 361005, P. R. China.
Lab Chip. 2020 Oct 7;20(19):3625-3632. doi: 10.1039/d0lc00505c. Epub 2020 Sep 9.
The enzyme-linked immunosorbent assay (ELISA) is one of the most commonly implemented clinical diagnostic tools for the detection and quantification of protein biomarkers. However, conventional ELISA tests require sophisticated infrastructure, expensive reagents, long assay time, and expertise for operation, which are not often easily accessible in resource-limited settings. Microfluidic ELISA-chip based point-of-care (POC) testing allows miniaturization and integration of complex functions that facilitate their usage in limited-resource settings. The current work demonstrates a simple, portable, low cost and equipment-free paper/poly(methyl methacrylate) (PMMA) integrated microfluidic ELISA-chip as a POC device with a visual distance-based readout for quantitative detection of clinical biomarkers. The integrated paper/PMMA ELISA-chip utilizes the movement of immunoassay complexes with magnetic beads by a permanent magnet in a PMMA part of the compartment. The target concentration is translated into a visual distance signal readout for quantitative detection of biomarkers in a μPAD. Because it does not require sophisticated instruments and has the added advantages of low cost, easy operation, and disposability with quantitative visual readout, the paper/PMMA ELISA-chip holds great promise for portable detection of target bioanalytes as a POC diagnostic tool in resource-limited setups.
酶联免疫吸附测定（ELISA）是检测和定量蛋白质生物标志物最常用的临床诊断工具之一。然而，传统的ELISA检测需要复杂的基础设施、昂贵的试剂、较长的检测时间以及操作所需的专业知识，而在资源有限的环境中这些条件往往不易具备。基于微流控ELISA芯片的即时检测（POC）能够实现复杂功能的小型化和集成化，便于在资源有限的环境中使用。当前的工作展示了一种简单、便携、低成本且无需设备的纸质/聚甲基丙烯酸甲酯（PMMA）集成微流控ELISA芯片，作为一种POC设备，具有基于视觉距离的读数，用于定量检测临床生物标志物。集成的纸质/PMMA ELISA芯片利用永久磁铁使免疫分析复合物与磁珠在隔室的PMMA部分中移动。目标浓度被转化为视觉距离信号读数，用于在μPAD中定量检测生物标志物。由于它不需要复杂的仪器，并且具有低成本、操作简便以及可一次性使用并具备定量视觉读数等额外优点，纸质/PMMA ELISA芯片作为资源有限环境中的POC诊断工具，在便携式检测目标生物分析物方面具有巨大的潜力。
