Cagnani Giovana Rosso, Ibáñez-Redín Gisela, Tirich Beatriz, Gonçalves Débora, Balogh Debora T, Oliveira Osvaldo N
Sao Carlos Institute of Physics, University of Sao Paulo, 13560-970, São Carlos, São Paulo, Brazil.
Sao Carlos Institute of Physics, University of Sao Paulo, 13560-970, São Carlos, São Paulo, Brazil.
Biosens Bioelectron. 2020 Oct 1;165:112428. doi: 10.1016/j.bios.2020.112428. Epub 2020 Jul 4.
The manufacture of sensors using large-scale production techniques, such as roll-to-roll (R2R) processing, may fulfill requirements of low-cost disposable devices. Herein, we report the fabrication of fully-printed electrochemical sensors using screen-printed carbon electrodes coated with carbon black inks through slot-die coating within an R2R process. As a proof of concept, sensors were produced to detect the neurotransmitter dopamine with high reproducibility and low limit of detection (0.09 μmol L). Furthermore, fully-printed biosensors made with a tyrosinase-containing ink were used to detect catechol in natural water samples. Since slot-die deposition enables printing enzymes without significant activity loss, the biosensors exhibited high stability over a period of several weeks. Even more important, R2R slot-die coating may be extended to any type of sensors and biosensors with the possibility of large-scale manufacturing.
使用大规模生产技术（如卷对卷（R2R）工艺）制造传感器，可能满足低成本一次性设备的要求。在此，我们报告了在R2R工艺中通过狭缝模涂覆使用涂有炭黑墨水的丝网印刷碳电极制造全印刷电化学传感器。作为概念验证，生产出了具有高重现性和低检测限（0.09 μmol/L）的用于检测神经递质多巴胺的传感器。此外，用含酪氨酸酶的墨水制成的全印刷生物传感器被用于检测天然水样中的儿茶酚。由于狭缝模涂覆能够在不显著损失活性的情况下印刷酶，这些生物传感器在数周时间内表现出高稳定性。更重要的是，R2R狭缝模涂覆可以扩展到任何类型的传感器和生物传感器，并具有大规模制造的可能性。
