Wang Junjie, Liu Tanghui, Mahmmod Yasser S, Yang Zipeng, Tan Jiexing, Ren Zhaowen, Zhang Xirui, Yang Xiaoying, Zhang Xiu-Xiang, Yuan Zi-Guo
College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, Guangdong, China.
Key Laboratory of Zoonosis Prevention and Control of Guangdong Province, Guangzhou 510642, Guangdong, China.
Microorganisms. 2020 Jul 28;8(8):1136. doi: 10.3390/microorganisms8081136.
() infection in female mammals during pregnancy can result in poor pregnancy. Similarly, it can result in male reproductive disorders in male mammals. Although the testes and uterus have very different biological makeup, they are still both attacked by resulting in reproductive dysfunctions. We hypothesized that there are significant common genes in the testes and uterus that interact with . Finding out and studying these genes is vital to understand the infection mechanism of and the induced disease pathogenesis. To achieve this goal, we built a mice model of acute infection with and the testes and uterus of the mice were sequenced by RNA-Seq. A total of 291 and 679 significantly differently expressed genes (DEGs) were obtained from the testes and the uterus, respectively. In the Gene Ontology (GO) analysis, part of the DEGs in the testes and uterus were related to 35 GO functions. When compared with the KEGG database, seven pathways affecting both the testes and uterus during the course of infection were identified. In addition, Toxoplasmosis can significantly affect the expression of and Insc leading to negative outcomes in the host. On the other hand, the host regulates , , and to defend against infection.
雌性哺乳动物在怀孕期间感染（病原体名称未给出）可导致妊娠不良。同样，它可导致雄性哺乳动物出现生殖障碍。尽管睾丸和子宫的生物学构成非常不同，但它们仍都会受到（病原体名称未给出）的攻击，从而导致生殖功能障碍。我们假设在睾丸和子宫中存在与（病原体名称未给出）相互作用的重要共同基因。找出并研究这些基因对于理解（病原体名称未给出）的感染机制以及所引发疾病的发病机制至关重要。为实现这一目标，我们构建了（病原体名称未给出）急性感染的小鼠模型，并通过RNA测序对小鼠的睾丸和子宫进行了测序。分别从睾丸和子宫中获得了总共291个和679个显著差异表达基因（DEGs）。在基因本体论（GO）分析中，睾丸和子宫中的部分DEGs与35个GO功能相关。与KEGG数据库相比，确定了在（病原体名称未给出）感染过程中影响睾丸和子宫的七条途径。此外，弓形虫病可显著影响（基因名称未给出）和Insc的表达，从而在宿主体内导致负面结果。另一方面，宿主调节（基因名称未给出）、（基因名称未给出）和（基因名称未给出）以抵御（病原体名称未给出）感染。
