Deng Meng-Ling, Chen Jun-Rong, Yang Jian-Fa, Ma Jun, Shu Fan-Fan, Zou Feng-Cai, He Jun-Jun
Faculty of Animal Science and Technology, Yunnan Agricultural University, Kunming, China.
Key Laboratory of Veterinary Public Health of Yunnan Province, College of Veterinary Medicine, Yunnan Agricultural University, Kunming, China.
Front Microbiol. 2024 Jun 28;15:1431183. doi: 10.3389/fmicb.2024.1431183. eCollection 2024.
is an obligate intracellular parasite of phylum Apicomplexa that poses a huge threat to pregnant hosts, and induces tragic outcomes for pregnant hosts, fetuses and newborns. However, the molecular mechanism underlying the tragic consequences caused by remains to be revealed. In the present study, we applied RNA-seq to study the transcriptomic landscape of the whole reproductive organ of pregnant mice post infection, aiming to reveal the key altered biological characters of reproductive organs of pregnant mice that could contribute to the tragic outcomes caused by infection. The results of the present study showed that the transcriptome of reproductive organs of pregnant mice was significantly altered by infection. A total of 2,598 differentially expressed genes (DEGs) were identified, including 1,449 upregulated genes and 1,149 downregulated genes. Enrichment analysis of the DEGs showed that the significantly altered features of reproductive organs of pregnant mice were excessive inflammatory responses, downregulated metabolism processes, and congenital diseases. The chemotaxis of immune cells in the reproductive organs of infected pregnant mice could also be reshaped by 19 differentially expressed chemokines and 6 differentially expressed chemokine receptors that could contribute to the damages of reproductive organ in pregnant mice. Overall, the findings of present study may help to understand the pathogenic mechanism of the acute infection in reproductive organs of pregnant mice, and it could also help to improve toxoplasmosis therapeutics for pregnant individuals.
是顶复门的专性细胞内寄生虫，对怀孕宿主构成巨大威胁，并给怀孕宿主、胎儿和新生儿带来悲惨后果。然而，由其导致这些悲惨后果的分子机制仍有待揭示。在本研究中，我们应用RNA测序技术研究感染后怀孕小鼠整个生殖器官的转录组图谱，旨在揭示怀孕小鼠生殖器官关键的生物学特征改变，这些改变可能导致感染所引发的悲惨后果。本研究结果表明，感染显著改变了怀孕小鼠生殖器官的转录组。共鉴定出2598个差异表达基因（DEG），其中包括1449个上调基因和1149个下调基因。对这些差异表达基因的富集分析表明，怀孕小鼠生殖器官显著改变的特征包括过度的炎症反应、下调的代谢过程和先天性疾病。感染的怀孕小鼠生殖器官中免疫细胞的趋化作用也可由19个差异表达的趋化因子和6个差异表达的趋化因子受体重塑，这可能导致怀孕小鼠生殖器官的损伤。总体而言，本研究结果可能有助于理解怀孕小鼠生殖器官急性感染的致病机制，也有助于改善针对孕妇的弓形虫病治疗方法。
