Department of Environmental and Occupational Health, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, UPM, Serdang 43400, Selangor, Malaysia.
Environmental Health Research Cluster (EHRc), Environmental Healthcare Section, Institute of Medical Science Technology, Universiti Kuala Lumpur, Kajang 43000, Selangor, Malaysia.
Int J Environ Res Public Health. 2020 Jul 28;17(15):5413. doi: 10.3390/ijerph17155413.
To explore the inflammation phenotypes following indoor pollutants exposure based on marker expression on eosinophils and neutrophils with the application of chemometric analysis approaches.
A cross-sectional study was undertaken among secondary school students in eight suburban and urban schools in the district of Hulu Langat, Selangor, Malaysia. The survey was completed by 96 students at the age of 14 by using the International Study of Asthma and Allergies in Children (ISAAC) and European Community Respiratory Health Survey (ECRHS) questionnaires. The fractional exhaled nitric oxide (FeNO) was measured, and an allergic skin prick test and sputum induction were performed for all students. Induced sputum samples were analysed for the expression of CD11b, CD35, CD63, and CD66b on eosinophils and neutrophils by flow cytometry. The particulate matter (PM and PM), NO, CO, and formaldehyde were measured inside the classrooms.
Chemometric and regression results have clustered the expression of CD63 with PM, CD11b with NO, CD66b with FeNO levels, and CO with eosinophils, with the prediction accuracy of the models being 71.88%, 76.04%, and 76.04%, respectively. Meanwhile, for neutrophils, the CD63 and CD66b clustering with PM and CD11b with FeNO levels showed a model prediction accuracy of 72.92% and 71.88%, respectively.
The findings indicated that the exposure to PM and NO was likely associated with the degranulation of eosinophils and neutrophils, following the activation mechanisms that led to the inflammatory reactions.
应用化学计量分析方法，基于嗜酸性粒细胞和中性粒细胞表面标志物的表达，探索室内污染物暴露后的炎症表型。
在马来西亚雪兰莪州 Hulu Langat 区的 8 所郊区和城市学校中，对中学生进行了一项横断面研究。共有 96 名 14 岁的学生完成了国际儿童哮喘和过敏研究（ISAAC）和欧洲社区呼吸健康调查（ECRHS）问卷。对所有学生进行了呼出气一氧化氮（FeNO）测量、过敏皮肤点刺试验和痰诱导。通过流式细胞术分析诱导痰样本中嗜酸性粒细胞和中性粒细胞上 CD11b、CD35、CD63 和 CD66b 的表达。在教室内测量了颗粒物（PM 和 PM）、NO、CO 和甲醛。
化学计量学和回归结果将 CD63 与 PM、CD11b 与 NO、CD66b 与 FeNO 水平聚类，CO 与嗜酸性粒细胞聚类，模型的预测准确率分别为 71.88%、76.04%和 76.04%。同时，对于中性粒细胞，CD63 和 CD66b 与 PM 聚类，CD11b 与 FeNO 水平聚类，模型预测准确率分别为 72.92%和 71.88%。
研究结果表明，PM 和 NO 的暴露可能与嗜酸性粒细胞和中性粒细胞脱颗粒有关，这是导致炎症反应的激活机制所致。
