Mizusaki S, Okamoto H, Akiyama A, Fukuhara Y
Mutat Res. 1977 Jul;48(3-4):319-25. doi: 10.1016/0027-5107(77)90175-0.
Mutagenic activities of cigarette smoke condensate were assayed in the presence of S-9 Mix using Salmonella typhimurium TA 98. The results were examined in relation to chemical data of tobacco leaves. Among the nitrogenous constituents examined, the contents of total nitrogen and protein nitrogen and the soluble nitrogenous fraction were positively and significantly related to an increase in mutagenic activity of the smoke condensate, whereas nicotine and nitrate were not important in contributing to mutagenic potency of such condensates. The age of tobacco leaves influenced the mutagenic potency of the condensate, which was lowest in leaves from the lower stalk position and increased with ascending leaf position on the stalk. Smoke condensate from tobacco with higher sugar content resulted in lower mutagenic activity. The present results, together with the previous study on the mutagenicity of the amino acid pyrolyzates, suggest that potent mutagens in cigarette smoke condensate are nitrogen-containing compounds, which may be formed from proteins and amino acids during the burning of a cigarette.
在S-9混合液存在的情况下，使用鼠伤寒沙门氏菌TA 98检测香烟烟雾浓缩物的诱变活性。结合烟叶的化学数据对结果进行了研究。在所检测的含氮成分中，总氮、蛋白质氮和可溶性含氮部分的含量与烟雾浓缩物诱变活性的增加呈正相关且具有显著相关性，而尼古丁和硝酸盐对这种浓缩物的诱变效力贡献不大。烟叶的年龄影响浓缩物的诱变效力，茎下部位置的烟叶中诱变效力最低，且随着茎上叶片位置的上升而增加。含糖量较高的烟草产生的烟雾浓缩物诱变活性较低。目前的结果，连同之前关于氨基酸热解产物诱变性的研究表明，香烟烟雾浓缩物中的强效诱变剂是含氮化合物，它们可能是在香烟燃烧过程中由蛋白质和氨基酸形成的。
