Matsumoto T, Yoshida D, Mizusaki S, Okamoto H
Mutat Res. 1978 Jan;56(3):281-8. doi: 10.1016/0027-5107(78)90194-x.
Pyrolyzates of 10 peptides, 10 proteins and 5 naturally-occurring materials were tested for mutagenicity in the histidine-requiring mutants Salmonella typhimurium TA98 and TA100. Significant mutagenic activity was detected with pyrolyzates of most of these materials. The pyrolyzates requred a liver microsomal fraction, as representative of mammalian metabolism, for their detection as mutagens. Among the pyrolyzates tested, the highest mutagenic activity was observed with that of a tryptophan-containing peptide. The pyrolyzate of protein obtained from tobacco leaf also showed mutagenicity. The higher the protein content in the leaf the higher the mutagenic activity of the pyrolyzate. Protein in a tobacco leaf may be the principal precursor of mutagens in tobacco-smoke condensate.
对10种肽、10种蛋白质和5种天然物质的热解产物进行了测试，以检测其对需要组氨酸的鼠伤寒沙门氏菌TA98和TA100突变体的致突变性。这些物质中的大多数热解产物都检测到了显著的致突变活性。热解产物需要肝脏微粒体部分作为哺乳动物代谢的代表，才能被检测为诱变剂。在所测试的热解产物中，含色氨酸的肽的热解产物表现出最高的致突变活性。从烟草叶中获得的蛋白质热解产物也显示出致突变性。叶中的蛋白质含量越高，热解产物的致突变活性就越高。烟草叶中的蛋白质可能是烟草烟雾冷凝物中诱变剂的主要前体。
