Using accurate nutrient values for ingredients is of vital importance for efficient diet formulation. The net energy (NE) system accounts for the real available amount of feedstuff energy for body maintenance and production as it considers energy dissipated as heat increment. The NE content of diets for pigs and broilers has been estimated from their nutrient contents. However, such estimates have not been made specifically for laying hens. This study reports the development of equations to predict NE for laying hens based on the chemical composition of 16 different diets meeting minimum nutrient specifications but varying in nutrient composition. Heat production and energy metabolism were measured in layers ranging from 32 to 62 weeks of age in closed-circuit calorimetry chambers with 8 replicates per diet in a randomized design. Each replicate consisted of a chamber with 3 layers that were adapted for 4 D to diets and chambers prior to measurement. The measurements included feed intake, metabolizable energy (ME) content, nitrogen balance, egg production, gas exchange, heat production, energy efficiency, and energy partition for a 3-D period. The average AME/GE and NE/AME ratios of the 16 diets were 77 and 74%, respectively. The latter ratio increased with energy efficiency (EE) content and decreased with CP content of diets. The results indicate that diet NE content can be predicted from AME, CP, and EE contents and the NE/AME ratio varied positively with EE and negatively with CP. A validation experiment with 2 diets fed to layers in calorimetry chambers confirmed the estimation from NE prediction equations. In conclusion, NE of diets can be predicted in laying hens from equations based on AME and CP and EE contents in laying hens being similar to those reported in broilers.