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蜂房哈夫尼亚菌脂多糖:分离、糖组成及十二烷基硫酸钠-聚丙烯酰胺凝胶电泳分析

Hafnia alvei lipopolysaccharides: isolation, sugar composition and SDS-PAGE analysis.

作者信息

Romanowska A, Katzenellenbogen E, Kułakowska M, Gamian A, Witkowska D, Mulczyk M, Romanowska E

机构信息

Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Wrocław, Poland.

出版信息

FEMS Microbiol Immunol. 1988 Dec;1(3):151-5. doi: 10.1111/j.1574-6968.1988.tb02368.x.

DOI:10.1111/j.1574-6968.1988.tb02368.x
PMID:3273465
Abstract

Lipopolysaccharides (LPS) of 33 strains of Hafnia alvei were isolated and purified. LPS content of the dry bacterial mass ranged from 1.2 to 4.5%. All examined lipopolysaccharides contained glucose, glucosamine, heptose, 3-deoxy-octulosonic acid and often galactose. Rhamnose, mannose, galactosamine, mannosamine and unidentified amino sugars were found in some H. alvei strains. Sialic acid was present in LPS of one strain. D-3-Hydroxybutyryl groups also were identified in lipopolysaccharides of 5 strains of this genus. SDS-PAGE of the lipopolysaccharides was presented in the paper. According to these results two core types exist in H. alvei.

摘要

分离并纯化了33株蜂房哈夫尼亚菌的脂多糖(LPS)。干菌体的LPS含量在1.2%至4.5%之间。所有检测的脂多糖都含有葡萄糖、氨基葡萄糖、庚糖、3-脱氧辛糖酸,且通常含有半乳糖。在一些蜂房哈夫尼亚菌菌株中发现了鼠李糖、甘露糖、半乳糖胺、甘露糖胺和未鉴定的氨基糖。在一株菌的LPS中存在唾液酸。在该属的5株菌的脂多糖中也鉴定出了D-3-羟基丁酰基。本文展示了脂多糖的SDS-PAGE图谱。根据这些结果,蜂房哈夫尼亚菌存在两种核心类型。

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