Department of Anthropology, University of South Florida, Tampa, Florida, USA.
Am J Hum Biol. 2021 May;33(3):e23477. doi: 10.1002/ajhb.23477. Epub 2020 Jul 31.
This study assesses the feasibility of measuring interleukin-1β (IL-1β) and interleukin-1 receptor antagonist (IL-1ra) in infant salivary samples as representative of pro- and anti-inflammatory processes, and explores predictors of these biomarkers in a US population.
Data were collected from 73 US mother-infant pairs. Salivary samples were collected with an infant swab and analyzed for IL-1β, IL-1ra, and immunoglobulin A (IgA) using ELISA. Household, maternal, infant, and anthropometric predictors were selected using stepwise regression to build final multivariate models.
Both IL-1β and IL-1ra can be feasibly measured in infant saliva. The predictors in the final IL-1β model were IL-1ra and reported infant illness. IL-1β, IgA, infant age, household income, maternal BMI, and infant weight-for-age z-score were significant in the final model for IL-1ra.
IL-1β and IL-ra are useful biomarkers of immune function for infants. In particular, IL-1ra has the potential to address the relationship between immune function and body composition in the mother-infant dyad.
本研究评估了通过测量婴儿唾液样本中的白细胞介素-1β（IL-1β）和白细胞介素-1 受体拮抗剂（IL-1ra）来反映促炎和抗炎过程的可行性，并探讨了美国人群中这些生物标志物的预测因素。
从 73 对美国母婴对中收集数据。使用婴儿拭子采集唾液样本，并使用 ELISA 法分析 IL-1β、IL-1ra 和免疫球蛋白 A（IgA）。使用逐步回归选择家庭、产妇、婴儿和人体测量学预测因素，以建立最终的多变量模型。
IL-1β 和 IL-1ra 均可在婴儿唾液中进行可靠测量。IL-1β 模型中的最终预测因素是 IL-1ra 和报告的婴儿疾病。IL-1β、IgA、婴儿年龄、家庭收入、产妇 BMI 和婴儿体重-年龄 z 评分在 IL-1ra 的最终模型中具有显著意义。
IL-1β 和 IL-ra 是婴儿免疫功能的有用生物标志物。特别是，IL-1ra 有可能解决母婴对子代免疫功能和身体成分之间的关系。
