Key Laboratory of Tropical Fruit Biology of Ministry of Agriculture, South Subtropical Crops Research Institute, Chinese Academy of Tropical Agricultural Sciences, Zhanjiang 524091, China.
Haikou Experimental Station (Institute of Tropical Fruit Tree), Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China.
Genomics. 2020 Nov;112(6):4505-4515. doi: 10.1016/j.ygeno.2020.07.038. Epub 2020 Jul 28.
Temporal transcriptome analysis combined with targeted metabolomics was employed to investigate the mechanisms of high sugar accumulation in fruit pulp of two contrasting mango cultivars. Ten sugar metabolites were identified in mango pulp with the most dominant being d-glucose. Analysis of the gene expression patterns revealed that the high-sugar cultivar prioritized the conversion of sucrose to d-glucose by up-regulating invertases and β-glucosidases and increased other genes directly contributing to the synthesis of sucrose and d-glucose. In contrast, it repressed the expression of genes converting sucrose, d-glucose and other sugars into intermediates compounds for downstream processes. It also strongly increased the expression of alpha-amylases which may promote high degradation of starch into d-glucose. Besides, ¾ of the sugar transporters was strongly up-regulated, indicative of their preponderant role in sugar accumulation in mango fruit. Overall, this study provides a good insight into the regulation pattern of high sugar accumulation in mango pulp.
采用时间转录组分析结合靶向代谢组学的方法研究了两个不同芒果品种果肉中高糖积累的机制。在芒果果肉中鉴定出 10 种糖代谢物，其中最主要的是 d-葡萄糖。基因表达模式分析表明，高糖品种通过上调转化酶和β-葡萄糖苷酶优先将蔗糖转化为 d-葡萄糖，并增加了其他直接参与蔗糖和 d-葡萄糖合成的基因。相比之下，它抑制了将蔗糖、d-葡萄糖和其他糖转化为下游过程中间化合物的基因表达。它还强烈增加了α-淀粉酶的表达，这可能促进淀粉强烈降解为 d-葡萄糖。此外，¾的糖转运蛋白强烈上调，表明它们在芒果果实糖积累中起主导作用。总的来说，这项研究深入了解了芒果果肉中高糖积累的调控模式。
