School of Biosciences, Faculty of Science, The University of Nottingham, Malaysia Campus, Jalan Broga, Semenyih, Selangor Darul Ehsan, 43500, Malaysia.
Agro-Biotechnology Institute Malaysia (ABI), National Institutes of Biotechnology Malaysia (NIBM) c/o MARDI Headquarters, 43400, Serdang, Selangor, Malaysia.
Protein J. 2019 Dec;38(6):704-715. doi: 10.1007/s10930-019-09868-x.
Mango (Mangifera indica L.) is an economically important fruit. However, the marketability of mango is affected by the perishable nature and short shelf-life of the fruit. Therefore, a better understanding of the mango ripening process is of great importance towards extending its postharvest shelf life. Proteomics is a powerful tool that can be used to elucidate the complex ripening process at the cellular and molecular levels. This study utilized 2-dimensional gel electrophoresis (2D-GE) coupled with MALDI-TOF/TOF to identify differentially abundant proteins during the ripening process of the two varieties of tropical mango, Mangifera indica cv. 'Chokanan' and Mangifera indica cv 'Golden Phoenix'. The comparative analysis between the ripe and unripe stages of mango fruit mesocarp revealed that the differentially abundant proteins identified could be grouped into the three categories namely, ethylene synthesis and aromatic volatiles, cell wall degradation and stress-response proteins. There was an additional category for differential proteins identified from the 'Chokanan' variety namely, energy and carbohydrate metabolism. However, of all the differential proteins identified, only methionine gamma-lyase was found in both 'Chokanan' and 'Golden Phoenix' varieties. Six differential proteins were selected from each variety for validation by analysing their respective transcript expression using reverse transcription-quantitative PCR (RT-qPCR). The results revealed that two genes namely, glutathione S-transferase (GST) and alpha-1,4 glucan phosphorylase (AGP) were found to express in concordant with protein abundant. The findings will provide an insight into the fruit ripening process of different varieties of mango fruits, which is important for postharvest management.
芒果（Mangifera indica L.）是一种经济上重要的水果。然而，由于果实易腐和货架期短，芒果的市场销售受到影响。因此，更好地了解芒果的成熟过程对于延长其采后货架期非常重要。蛋白质组学是一种强大的工具，可以用于阐明细胞和分子水平上复杂的成熟过程。本研究利用 2 维凝胶电泳（2D-GE）结合 MALDI-TOF/TOF 技术，鉴定了两个热带芒果品种（Mangifera indica cv. 'Chokanan'和 Mangifera indica cv 'Golden Phoenix'）在成熟过程中差异丰度的蛋白质。对芒果果实中果皮的成熟和未成熟阶段进行比较分析表明，鉴定出的差异丰度蛋白质可分为三个类别，即乙烯合成和芳香挥发物、细胞壁降解和应激响应蛋白。'Chokanan'品种还鉴定出一个差异蛋白类别，即能量和碳水化合物代谢。然而，在所鉴定的所有差异蛋白中，只有蛋氨酸 γ-裂解酶在'Chokanan'和'Golden Phoenix'品种中都有发现。从每个品种中选择了 6 个差异蛋白进行验证，方法是使用反转录定量 PCR（RT-qPCR）分析它们各自的转录表达。结果表明，有两个基因，谷胱甘肽 S-转移酶（GST）和α-1,4 葡聚糖磷酸化酶（AGP）的表达与蛋白丰度一致。这些发现将为不同品种芒果果实的成熟过程提供深入了解，这对于采后管理非常重要。
