Evans T L, Miller R A
IDEC Pharmaceuticals Corp., Mountain View, CA 94043.
Biotechniques. 1988 Sep;6(8):762-7.
We have produced large quantities of murine monoclonal antibodies for in vivo human clinical trials using hollow fiber bioreactors (HFBRs). Thirty-three different hybridoma cell lines have been evaluated in various HFBR systems. Monoclonal antibody (Ab) productivity is highly dependent on the intrinsic secretory rate of each cell line. Other factors that affect Ab production include capillary membrane molecular weight cutoff, and HFBR design. Studies comparing HFBRs to static and suspension culture systems revealed similar Ab productivity. An advantage of the HFBR is that the Ab is concentrated in the extracapillary space, simplifying downstream processing.
我们已使用中空纤维生物反应器（HFBRs）生产了大量用于人体临床试验的鼠单克隆抗体。已在各种HFBR系统中评估了33种不同的杂交瘤细胞系。单克隆抗体（Ab）的生产力高度依赖于每个细胞系的内在分泌率。影响Ab产生的其他因素包括毛细血管膜的截留分子量和HFBR设计。将HFBR与静态和悬浮培养系统进行比较的研究表明，Ab生产力相似。HFBR的一个优点是Ab集中在毛细血管外空间，简化了下游加工。
