Oh D J, Choi S K, Chang H N, Choe T B
Bioprocess Engineering Research Center, Korea Advanced Institute of Science and Technology, Taejon.
Cytotechnology. 1993;13(1):41-9. doi: 10.1007/BF00749974.
A new method of making microcapsules with calcium alginate gel was developed and the cultivation of the encapsulated hybridoma cells producing monoclonal antibodies against human chorionic gonadotropin was investigated. A high cell density of 2.0 x 10(8) cells/cm3 in the capsules led to a high dilution rate of 0.67 per hour and resulted in the high volumetric monoclonal antibody productivity of 652.8 mg/l/day, which was 20-30 times higher than those of traditional continuous suspension cultures. However, long-term continuous culture was not achieved with this capsule system probably because of the limitation in nutrient supply and the accumulation of waste products. Also the analysis of oxygen transfer in this system showed that oxygen supply was not enough to support such a high cell density.
开发了一种用海藻酸钙凝胶制备微胶囊的新方法，并对包封的产生抗人绒毛膜促性腺激素单克隆抗体的杂交瘤细胞的培养进行了研究。微胶囊中2.0×10⁸个细胞/cm³的高细胞密度导致每小时0.67的高稀释率，并产生了652.8mg/l/天的高体积单克隆抗体生产率，这比传统的连续悬浮培养高20 - 30倍。然而，这种胶囊系统未能实现长期连续培养，可能是由于营养供应的限制和废物的积累。此外，该系统中的氧传递分析表明，氧气供应不足以支持如此高的细胞密度。
