White L A, Ades E W
Center for Infectious Diseases, Centers for Disease Control, Atlanta, Georgia 30333.
J Clin Microbiol. 1990 Feb;28(2):283-6. doi: 10.1128/jcm.28.2.283-286.1990.
Minimum essential medium supplemented with either 7% fetal bovine serum or 2% fetal bovine serum and 1.5% low protein serum replacement (Sigma Chemical Co., St. Louis, Mo.) factor was used to evaluate the growth of Vero 76 cells (E-6 clone) in a cell culture bioreactor. Three different microcarriers, collagen-coated polystyrene (Kontes Life Sciences Products, Vineland, N.J.), Gelibead (Hazelton Research Products, Inc., Lenexa, Kans.), and Cytodex 3 (Pharmacia Fine Chemicals, Piscataway, N.J.), were used to compare the propagation of these anchorage-dependent cells. The number of viable cells recovered during three harvests over an 11-day incubation period was evaluated. The Vero E-6 cells grew equally well with both media. When the collagen-coated dextran microcarriers (Cytodex 3) were used, better cell attachment and higher-density growth were obtained and 5.5 x 10(8) to 7.0 x 10(8) cells were recovered from the system.
用补充了7%胎牛血清或2%胎牛血清与1.5%低蛋白血清替代物（西格玛化学公司，密苏里州圣路易斯）的最低必需培养基来评估非洲绿猴肾细胞（Vero 76细胞，E-6克隆）在细胞培养生物反应器中的生长情况。使用了三种不同的微载体，即胶原包被的聚苯乙烯（康宁生命科学产品公司，新泽西州文兰）、Gelibead（黑泽尔顿研究产品公司，堪萨斯州莱尼克斯）和Cytodex 3（法玛西亚精细化学品公司，新泽西州皮斯卡塔韦），以比较这些贴壁依赖性细胞的增殖情况。评估了在11天培养期内三次收获时回收的活细胞数量。Vero E-6细胞在两种培养基中生长情况相同。当使用胶原包被的葡聚糖微载体（Cytodex 3）时，细胞贴壁更好，生长密度更高，从该系统中回收了5.5×10⁸至7.0×10⁸个细胞。
