GOLGA4，一种高尔基体基质蛋白，在小鼠的精子发生和雄性生育力中可有可无。
GOLGA4, A Golgi matrix protein, is dispensable for spermatogenesis and male fertility in mice.
机构信息
Institute Reproductive Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, 430030, China.
Institute Reproductive Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, 430030, China; Shenzhen Huazhong University of Science and Technology Research Institute, Shenzhen, Guangdong, 518057, China.
出版信息
Biochem Biophys Res Commun. 2020 Aug 27;529(3):642-646. doi: 10.1016/j.bbrc.2020.05.170. Epub 2020 Jul 18.
During acrosome biogenesis, numerous granules formed from trans-Golgi stacks and accumulated in the concave region of the nuclear surface that is essential for acrosome formation. Several Golgi-associated proteins were involved in this process. However, the specific function of Golgi-associated proteins, especially Golgi matrix protein, during acrosome biogenesis remains elusive. In this study, we identified GOLGA4, as a Golgi matrix protein, highly expressed in mouse testes. To explore the function of GOLGA4 in spermatogenesis, we generated a Golga4 global knockout mouse line using CRISPR/Cas9 technology and demonstrated that Golga4 knockout males are fertile with normal morphology of testis and sperm. Furthermore, testicular histology showed no significant difference between WT and KO mice. Together, our data demonstrate that GOLGA4 is dispensable for mouse spermatogenesis and male fertility.
在顶体生物发生过程中，许多颗粒从跨高尔基堆叠形成并积累在核表面的凹陷区域，这对顶体的形成至关重要。有几个与高尔基体相关的蛋白质参与了这个过程。然而，高尔基体相关蛋白，特别是高尔基体基质蛋白，在顶体生物发生过程中的具体功能仍然难以捉摸。在这项研究中，我们鉴定了 GOLGA4 作为一种高尔基体基质蛋白，在小鼠睾丸中高度表达。为了探索 GOLGA4 在精子发生中的功能，我们使用 CRISPR/Cas9 技术生成了一个 Golga4 全局敲除小鼠品系，并证明 Golga4 敲除雄性具有正常形态的睾丸和精子，具有正常的生育能力。此外，睾丸组织学显示 WT 和 KO 小鼠之间没有显著差异。总之，我们的数据表明 GOLGA4 对于小鼠精子发生和雄性生育能力不是必需的。
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