WDFY1，一种 WD40 重复蛋白，在小鼠的精子发生和雄性生育力中不是必需的。
WDFY1, a WD40 repeat protein, is not essential for spermatogenesis and male fertility in mice.
机构信息
Institute of Reproductive Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China; Shenzhen Key Laboratory for Neuronal Structural Biology, Biomedical Research Institute, Shenzhen Peking University-The Hong Kong University of Science and Technology Medical Center, Shenzhen, 518036, China.
Institute of Reproductive Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China.
出版信息
Biochem Biophys Res Commun. 2022 Mar 12;596:71-75. doi: 10.1016/j.bbrc.2022.01.084. Epub 2022 Jan 28.
The mouse WD repeat and FYVE domain containing 1 (Wdfy1) gene is located in chromosome 1qC4 and spans over 73.7 kilobases. It encodes a protein of 410-amino acid protein that shares 97.8% amino acid sequence identity with the human WDFY1 protein. However, the expression pattern of WDFY1 in reproductive organs and its function in male fertility remain unknown. In this study, we generated transgenic mice expressing FLAG-Wdfy1-mCherry cDNA driven by the Wdfy1 promoter to clarify the expression of WDFY1. The results showed that WDFY1 is highly expressed in mouse testes and located in the cytoplasm of late pachytene spermatocytes to elongated spermatids. Interestingly, the global Wdfy1 knockout (KO) male mice displayed normal growth, development, and fertility. Further histological analysis of Wdfy1 knockout mouse testes revealed that all spermatogenic cells are present in Wdfy1 KO seminiferous tubules. Together, our data demonstrate that WDFY1 is dispensable for mouse spermatogenesis and male fertility.
鼠 WD 重复和 FYVE 结构域包含 1 号基因（Wdfy1）位于 1qC4 染色体上，跨越超过 73.7 千碱基。它编码一个 410 个氨基酸的蛋白质，与人类 WDFY1 蛋白具有 97.8%的氨基酸序列同一性。然而，WDFY1 在生殖器官中的表达模式及其在男性生育力中的功能仍然未知。在这项研究中，我们生成了表达由 Wdfy1 启动子驱动的 FLAG-Wdfy1-mCherry cDNA 的转基因小鼠，以阐明 WDFY1 的表达。结果表明，WDFY1 在小鼠睾丸中高度表达，位于晚期粗线期精母细胞到伸长的精子细胞的细胞质中。有趣的是，Wdfy1 敲除（KO）雄性小鼠表现出正常的生长、发育和生育能力。对 Wdfy1 KO 睾丸的进一步组织学分析表明，所有精原细胞都存在于 Wdfy1 KO 曲细精管中。总之，我们的数据表明 WDFY1 对于小鼠精子发生和雄性生育力不是必需的。
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