Corrosion Science and Technology Division, Metallurgy and Materials Group Indira Gandhi Centre for Atomic Research, Kalpakkam, 603102, India.
Corrosion Science and Technology Division, Metallurgy and Materials Group Indira Gandhi Centre for Atomic Research, Kalpakkam, 603102, India.
Anal Chim Acta. 2020 Aug 22;1126:38-51. doi: 10.1016/j.aca.2020.05.072. Epub 2020 Jun 21.
In the present study, the efficacies of three different cationic and anionic ionic liquids (ILs) on biofilm formation on materials used in cooling water systems were evaluated. Two imidazolium based ILs; 1-Ethyl 3-Methylimidazolium tetrafluoroborate - (IL-E) and 1-Butyl-3-methylimidazolium chloride - (IL-I) with anionic fluoride and chloride groups and one piperidinium based IL, N-methyl-N-propylpiperidinium bis(trifluoromethylsulfonyl)imide - (IL-M) with fluoromethyl group as anion were used. The efficacy of these ILs were evaluated on planktonic and sessile cells of major biofilm formers in cooling water systems using Gram negative bacterium Pseudomonas sp. and Gram positive bacterium Bacillus sp. Further their effect on inhibiting biofilm formation on titanium and carbon steel surfaces were also evaluated. Results showed that planktonic cells of Pseudomonas sp. and Bacillus sp. were effectively inhibited by 25 ppm of IL-M and IL-E, respectively. For both bacteria, 50 ppm of IL-I was enough to inhibit and eradicate the sessile cell formation. Among the three ILs, IL-E was the best in inhibiting the adhesion of bacterial cells on Ti and CS surfaces. These results suggest that Imidazolium based ILs are effective in controlling sessile cell formation and eradicating mature biofilm as compared to piperidinium based IL. Further, Imidazolium based IL with fluoride anion (IL-E) was the best in inhibiting adhesion of these bacterial cells and thereby biofilm formation on material surfaces. This study establishes the feasibility of using ILs in cooling water system for bacterial biofilm control along with other conventional biofouling control methods.
在本研究中，评估了三种不同阳离子和阴离子离子液体（ILs）对冷却水系统中使用的材料上生物膜形成的功效。两种基于咪唑啉的 IL；具有阴离子氟化物和氯化物基团的 1-乙基-3-甲基咪唑四氟硼酸盐-（IL-E）和 1-丁基-3-甲基咪唑氯化物-（IL-I），以及一种基于哌啶的 IL，N-甲基-N-丙基哌啶双（三氟甲基磺酰基）亚胺-（IL-M），其阴离子为氟甲基。使用革兰氏阴性菌假单胞菌和革兰氏阳性菌芽孢杆菌来评估这些 IL 对浮游和静止细胞的功效。进一步评估了它们对抑制钛和碳钢表面生物膜形成的影响。结果表明，浮游细胞假单胞菌和芽孢杆菌分别被 25 ppm 的 IL-M 和 IL-E 有效抑制。对于这两种细菌，50 ppm 的 IL-I 足以抑制和消除浮游细胞的形成。在三种 IL 中，IL-E 抑制细菌细胞在 Ti 和 CS 表面附着的效果最好。这些结果表明，与基于哌啶的 IL 相比，基于咪唑啉的 IL 可有效控制浮游细胞的形成并根除成熟的生物膜。此外，具有氟阴离子（IL-E）的基于咪唑啉的 IL 最能抑制这些细菌细胞的粘附，从而抑制材料表面的生物膜形成。本研究确立了在冷却水系统中使用 IL 与其他常规生物污垢控制方法一起控制细菌生物膜的可行性。
